华北农学报2025，Vol.40Issue(5)：26-34,9.DOI:10.7668/hbnxb.20195799
氨基酸透性酶基因OsAAP8超表达对水稻生长发育及其稻米品质的影响
Effects of Amino Acid Permease Gene OsAAP8 Overexpression on Rice Growth and Quality
摘要
Abstract
OsAAP8 overexpressed transgenic lines were constructed based on the japonica rice variety Zhonghua 11.By detecting and analyzing its agronomic and quality traits,it explored the effects of OsAAP8 overexpression on rice growth and development,as well as rice quality,to provide theoretical basis for molecular design breeding by u-tilizing OsAAP8 gene to improve rice quality and yield.The results showed that compared with the transgenic nega-tive plants,OsAAP8 overexpressed transgenic positive plants had significantly reduced plant height,tiller number,and single plant yield.The quality trait testing results showed that the content of glutamic acid,threonine,essential amino acids,total amino acids,protein,amylose,gel consistency,and gelatinization temperature in OsAAP8 overex-pressed transgenic positive rice were significantly increased.The content of total starch and brown rice rate did not change significantly.The content of free fatty acids,taste value,polished rice rate,and whole polished rice rate were significantly decreased.The observation results of optical microscope and scanning electron microscope showed that the chalkiness rate and chalkiness degree of OsAAP8 overexpressed transgenic positive rice were significantly in-creased,but the chalkiness area did not change significantly,and the shape and arrangement of starch granules did not change.The grain size detection results showed no significant changes in grain length,width,and thickness of OsAAP8 overexpressed transgenic positive rice.The above results indicate that overexpression of OsAAP8 is not con-ducive to the growth and development of rice,but can significantly improve the nutritional quality of rice,which pro-vides important information for the cultivation of new high-quality rice varieties.关键词
水稻/氨基酸透性酶/OsAAP8/稻米品质/超表达Key words
Rice/Amino acid permease/OsAAP8/Rice quality/Overexpression分类
农业科技引用本文复制引用
彭波,申关望,祁玉良,汪全秀,孙艳芳,刘岩,徐茹欣,徐海芳,彭娟,赵强,张巧玉,黄雅琴,张志国..氨基酸透性酶基因OsAAP8超表达对水稻生长发育及其稻米品质的影响[J].华北农学报,2025,40(5):26-34,9.基金项目
国家自然科学基金项目(U2004141 （）
12305399) （）
河南省科技研发计划联合基金(应用攻关)项目(242103810019) （应用攻关）
河南省科技攻关项目(242102110266 （）
242102110270 （）
231111110500) （）
信阳市科技攻关项目(20230025) （20230025）
信阳师范大学大学生科研基金项目(202410477040) （202410477040）
