华北农学报2025，Vol.40Issue(5)：35-46,12.DOI:10.7668/hbnxb.20195612
花魔芋AkTCP家族基因鉴定及表达分析
Genomic Identification and Expression Analysis of TCP Family Genes in Amorphophallus konjac
摘要
Abstract
The TCP transcription factor family is unique to plants and plays a crucial role in key biological processes such as plant growth,metabolic activities,and stress responses.To investigate the quantity,distribution and expression patterns of TCP gene family in the Amorphophallus konjac genome,bioinformatics approaches were u-tilized to identify the AkTCP gene family,followed by a comprehensive analysis of the physicochemical properties,subcellular localization,collinearity,gene structure,and evolutionary relationships.Additionally,gene expression in response to biotic and abiotic stresses was examined by qRT-PCR.The findings were summarized as follows:the A.konjac genome contained 30 AkTCP family genes,with protein lengths ranging from 135 to 562 amino acids.These proteins had molecular weights between 15.08 and 57.20 ku,and isoelectric points between 4.96 and 11.49.Most of these were basic,hydrophilic,and unstable proteins,predominantly localized in the nucleus.These genes were un-evenly distributed across the chromosomes.The collinear AkTCP genes were unevenly distributed across eight chro-mosomes,comprising four inter-chromosomal and five intrachromosomal collinearity events.The AkTCP gene struc-ture was relatively simple,with most genes lacking introns,and all AkTCP transcription factors exhibit a highly con-served TCP domain.Phylogenetic classification separates the 30 AkTCP genes into two major classes,Class Ⅰ and Class Ⅱ,with Class Ⅱ further subdivided into the CIN and CYC/TB1 subclasses.The promoters of AkTCP genes contain cis-elements related to physiological response,light-responsive,phytohormone-responsive and stress-respon-sive elements.The results of qRT-PCR analysis indicated that AkTCP family members were involved in and respon-ded positively to drought stress,salt stress,methyl jasmonate and soft rot pathogens of A.konjac.关键词
花魔芋/TCP基因家族/转录因子/表达分析Key words
Amorphophallus konjac/TCP gene family/Transcription factor/Expression analysis分类
园艺学与植物营养学引用本文复制引用
何斐,汤鑫塬,李竹梅,褚洪龙..花魔芋AkTCP家族基因鉴定及表达分析[J].华北农学报,2025,40(5):35-46,12.基金项目
国家自然科学基金项目(31901468 （）
1860057) （）
云南省科技厅基础研究专项面上项目(202401AT070002) （202401AT070002）
安康市科技计划项目(AK2022-NY-04) （AK2022-NY-04）
安康学院专项(2023AYKCYZ05 （）
2024AYXNZX04) （）
曲靖师范学院博士创新团队支持计划项目 （）
