华北农学报2025，Vol.40Issue(5)：47-54,8.DOI:10.7668/hbnxb.20195591
基于转录组分析挖掘影响甘蓝型油菜含油量积累的候选基因
Transcriptome Analysis Reveal Candidate Genes Affecting Seed Oil Accumulation in Brassica napus
摘要
Abstract
In order to clarify the regulatory network of oil accumulation in Brassica napus and breed oilseed rapeseed varieties with high oil content.The seed transcriptome data of four rapeseed inbred lines at 25,35,and 45 days after flowering were used to identify candidate genes affecting oil content by transcriptome analysis and correla-tion analysis.Consequently,a total of 1 530 genes were identified exhibiting differential expression across all three period,comprising 986 up-regulated genes and 544 down-regulated genes.GO enrichment analysis of these differen-tially expressed genes detected 83 lipid biosynthesis genes,79 lipid degradation genes,21 lipid transporter genes and 80 transcription factors.To further analysis of these differentially expressed transcription factors,genes including BnTT8,BnGL2,and BnNAC082 were identified.Combined with the oil content data of 50 semi-winter rapeseed in three different regions over two years,four SNPs were identified in the exons region of the BnNAC082-A03 signifi-cantly associated with oil content using genome-wide association studies.Two haplotypes were detected in the region of this gene and BnNAC082-A03_Hap1 corresponding accessions showed significantly higher oil content than that of Hap2.Additionally,it utilized these transcriptomic data to construct co-expression analysis network,and in the sub-network revealed that BnNAC082-A03 was directly connected with BnTT8-A09 and BnGL2-C06,forming a potential molecular regulatory network affecting seed lipid accumulation.关键词
甘蓝型油菜/含油量/转录组分析/共表达网络/全基因组关联分析Key words
Brassica napus/Oil content/Transcriptome analysis/Co-expression networks/GWAS分类
农业科学引用本文复制引用
李文,姚敏,贺丹,邱萍,何昕,熊兴华,刘忠松,钱论文..基于转录组分析挖掘影响甘蓝型油菜含油量积累的候选基因[J].华北农学报,2025,40(5):47-54,8.基金项目
湖南科技创新领军人才项目(2023RC1063) （2023RC1063）
湖南省杰出青年科学基金项目(2022JJ10027) （2022JJ10027）
