华北农学报2025，Vol.40Issue(5)：55-61,7.DOI:10.7668/hbnxb.20195685
扁蓿豆MrAGL8基因的克隆、亚细胞定位及表达分析
Cloning,Subcellular Localization and Expression Analysis of MrAGL8 Gene in Medicago ruthenica
摘要
Abstract
In order to explore the relationship between MrAGL8 gene and pod dehiscence traits in Medicago ru-thenica.In this study,Medicago ruthenica was used as the plant material.The MrAGL8 gene was amplified by PCR,cloned,and sequenced.Additionally,bioinformatics analysis,subcellular localization,and expression analysis in dif-ferent tissues and organs were performed for this gene.The results showed that the complete coding region of MrA-GL8 cDNA with a length of 711 bp was obtained through cloning using PCR amplification technology.Bioinformatics analysis results showed that MrAGL8 encoded 236 amino acids,it had MADS-box and K-box protein conserve do-mains.Its molecular weight was 27.39 ku,the theoretical isoelectric point was 8.69,the total number of positively charged residues was 39,the total number of negatively charged residues was 36,and the instability coefficient was 48.5.The secondary structure of the protein contained α-helix and β-sheet.It was an unstable protein and belonged to a hydrophilic alkaline protein.Subcellular localization results showed that MrAGL8 protein was located in the nucleus.The expression analysis of different tissues and organs showed that the expression level of MrAGL8 in different tissues was stem＞root＞pod＞leaf＞flower,and the expression levels of roots and stems were significantly different from those of leaves,flowers and pods.The results showed that MrAGL8 gene was related to pod dehiscence.关键词
扁蓿豆/MrAGL8/基因克隆/亚细胞定位/表达分析Key words
Medicago ruthenica/MrAGL8/Gene cloning/Subcellular localization/Expression analysis分类
农业科技引用本文复制引用
于佳,郭慧琴,李宇霞,雷慧,任卫波..扁蓿豆MrAGL8基因的克隆、亚细胞定位及表达分析[J].华北农学报,2025,40(5):55-61,7.基金项目
内蒙古自治区种业科技创新重大示范工程"揭榜挂帅"项目(2022JBGS0040) （2022JBGS0040）
"科技兴蒙"重点专项(2020-科技兴蒙-草种业技术创新中心-2) （2020-科技兴蒙-草种业技术创新中心-2）
