华北农学报2025，Vol.40Issue(5)：62-73,12.DOI:10.7668/hbnxb.20195590
薄皮甜瓜果实香味差异的关键酯类物质鉴定及相关酶基因家族筛选
Identification of Key Ester Substances and Screening of Enzyme Gene Family Members for Aroma Differences in Fruits of Oriental Melons
摘要
Abstract
To determine the relationship between the esters compounds and key enzyme activities and genes that involved in the differences in aromas of oriental melon fruits,the disparities of ester metabolites and the enzyme activities and gene expression characteristics of CXE and AAT were investigated,with the fruits of oriental melon,DX108 and DX3-5,used as experimental materials.The results showed that a total of 644 metabolites were detected in oriental melon fruits,including 114 esters.A total of 108 metabolite variations and 23 different esters were detec-ted in mature fruits.Compared with DX3-5,the types and contents of esters in ripe fruits of DX108 increased signifi-cantly,indicating that esters were the key substances in the formation of aroma difference in ripe fruits of oriental melons.K-means clustering analysis showed that isoamyl acetate,ethyl 2-methybuyrate,β-ethyl phenylacetate,p-cresol acetate,hexyl acetate ester,methyl phenylacetate,methyl anthranilate and ethyl caproate were the key esters,which caused the difference of ripe fruit aroma between two oriental melons.In addition,the activities of AAT and CXE in fruits showed opposite trend,suggesting that the coordination of AAT and CXE affected the metabolism of esters.The expression characteristics of AAT and CXE gene family members were different with fruit development,and correlation analysis suggested that CmCXE5,CmCXE6 and CmAAT5 were involved in the metabolism of eight different esters in oriental melon ripe fruits.From those findings,the variations in synthesis and accumulation of eight key esters are important reasons that direct the difference in aroma of two kinds of oriental melon,and Cm-CXE5,CmCXE6 and CmAAT5 may play a major regulatory role.关键词
薄皮甜瓜/酯类物质/羧酸酯酶/醇酰基转移酶/基因表达Key words
Oriental melon/Esters/Carboxylesterase/Alcohol acyltransferase/Gene expression分类
园艺学与植物营养学引用本文复制引用
欧阳世贤,刘胜博,孙超,陈凯,何淑平,李春霞,靳亚忠..薄皮甜瓜果实香味差异的关键酯类物质鉴定及相关酶基因家族筛选[J].华北农学报,2025,40(5):62-73,12.基金项目
现代农业产业技术体系国家西甜瓜产业技术体系(CARS-25) （CARS-25）
农业生物经济协同创新推广体系 （）
黑龙江省自然科学基金项目(C2016039) （C2016039）
博士科研启动基金项目(XDB2015-01) （XDB2015-01）
黑龙江八一农垦大学三纵资助项目(ZRCPY202116) （ZRCPY202116）
