华北农学报2025，Vol.40Issue(5)：171-178,8.DOI:10.7668/hbnxb.20195821
StCAT1与StCAT3抗马铃薯早疫病的分子调控机制
The Molecular Regulatory Mechanism of StCAT1 and StCAT3 in Resistance to Early Blight in Potatoes
摘要
Abstract
Catalase(CAT)plays a critical role in plant resistance against biotic stresses.To investigate the func-tions of StCAT1 and StCAT3 in potato defense against Alternaria solani infection,this study cloned the cDNA se-quences of StCAT1 and StCAT3 via RT-PCR.Sequencing results demonstrated that both genes were 1 479 bp in length,encoding 493 amino acids.Phylogenetic tree construction revealed that StCAT1 shared high homology with SlCAT1 from tomato,while StCAT3 exhibited high homology with both SlCAT3(tomato)and SpCAT3 from wild to-mato.Following A.solani infection for 6 days,the expression of StCAT1 and StCAT3 was significantly upregulated by approximately 3.9-fold and 8.7-fold,respectively.A co-silencing vector targeting both StCAT1 and StCAT3 was con-structed,and transient co-silencing was achieved using virus-induced gene silencing(VIGS).qRT-PCR screening i-dentified an effective co-silenced potato line(VIGS-3)with suppressed StCAT1 and StCAT3 expression.Upon A.so-lani inoculation,the silenced line exhibited 42％larger lesion areas compared to the control,indicating that co-si-lencing StCAT1/StCAT3 compromised potato resistance to early blight.DAB(3,3'-diaminobenzidine)staining fur-ther demonstrated that H2O2 accumulation in co-silenced leaves was significantly elevated,with staining intensity be-ing 3.8-fold higher than that in the control.These results demonstrate that StCAT1 and StCAT3 regulate H2O2 home-ostasis to mediate potato resistance against A.solani,providing a theoretical foundation for elucidating the molecular mechanisms of CAT genes in potato-pathogen interactions.关键词
马铃薯早疫病/StCAT1/StCAT3/VIGS/H2O2Key words
Early Blight in Potatoes/StCAT1/StCAT3/VIGS/H2O2分类
农业科技引用本文复制引用
刘鑫,李倩,刘芳明,丁明亚,杨志辉,朱杰华..StCAT1与StCAT3抗马铃薯早疫病的分子调控机制[J].华北农学报,2025,40(5):171-178,8.基金项目
现代农业产业技术体系建设专项(CARS 09) （CARS 09）
国家重点研发计划项目(2024YFD1401204) （2024YFD1401204）
