华北农学报2025，Vol.40Issue(5)：231-238,8.DOI:10.7668/hbnxb.20195469
中国大鲵Galectin-3基因克隆、生物信息学及表达特性分析
Cloning,Bioinformatics and Expression Characteristics Analysis of Galectin-3 Gene in Chinese Giant Salamander
摘要
Abstract
In order to identify the molecular biological characteristics and expression patterns of Galectin-3 gene in Chinese giant salamander before and after infection,the Galectin-3 gene was amplified by RT-PCR,and its physi-cochemical properties and protein structure were analyzed by bioinformatics.Real-time Quantitative PCR(qRT-PCR)was used to detect the expression of Galectin-3 in different tissues of Chinese giant salamanders before and af-ter Aeromonas hydrophila.The results showed that the length of Galectin-3 gene CDS region was 351 bp,encoding 116 amino acids,the theoretical isoelectric point(pI)was 5.87,the relative molecular weight was 13.53 ku,and it was a stable hydrophilic protein located in the cytoplasm.Structural analysis showed that Galectin-3 protein con-tained a GLECT domain.The phylogenetic tree showed that the Galectin-3 amino acid sequence was closely related to Rhinatrema bivittatum.qRT-PCR results showed that Galectin-3 was expressed in all tested tissues of the salaman-der,and the relative expression level was the highest in the skin.The relative expression levels of Galectin-3 in the liver,muscle,intestines and kidneys of Chinese giant salamander increased significantly and reached the highest val-ue at 12 h of infection with A.hydrophila.The relative expression levels of Galectin-3 in the spleen and skin of Chi-nese giant salamander increased significantly and reached the highest value at 48 h of infection with A.hydrophila.In conclusion,Galectin-3 gene was actively expressed in the early stage of salamander infected by foreign bacteria,suggesting that Galectin-3 gene may be involved in the immune regulation of salamander.关键词
大鲵/Galectin-3/生物信息学分析/实时荧光定量PCR/嗜水气单胞菌Key words
Andrias davidianus/Galectin-3/Bioinformatics analysis/Real-time Fluorescence Quantitative PCR/Aeromonas hydrophila分类
水产学引用本文复制引用
商文聪,李灿,黎琴容,吴飞凡,苏淑贤,骆建林,王开卓,王慧,孟永禄,叶婷..中国大鲵Galectin-3基因克隆、生物信息学及表达特性分析[J].华北农学报,2025,40(5):231-238,8.基金项目
贵州省基础研究计划项目(黔科合基础-ZK[2022]一般011) （黔科合基础-ZK[2022]一般011）
贵阳学院博士科研启动基金项目(2019039510819) （2019039510819）
贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2020]091) （黔教合KY字[2020]091）
中央财政引导地方科技发展专项(QKZYD[2022]4013) （QKZYD[2022]4013）
贵州省科技厅贵州省山地珍稀动物与经济昆虫重点实验室项目(k18200006) （k18200006）
