哈尔滨医药2025，Vol.45Issue(5)：1-5,5.DOI:10.3969/j.issn.1001-8131.2025.05.001
无机硒对大鼠代谢性骨病的影响
To Investigate the Effect of Inorganic Selenium on Metabolic Bone Disease in Rats
摘要
Abstract
Objective To explore the effects of different selenium levels on metabolic bone disease in rats and observe the response after vitamin D treatment.Methods Rats were randomly divided into a blank control group,a non selenium supplemented group,a low selenium group,and a high selenium group.There were 30 rats in the blank control group,32 rats in the non selenium supplemented group,42 rats in the low selenium group,and 42 rats in the high selenium group.Except for the blank control group,the other three groups of rats were modeled using hydroxyethylenediphosphonic acid.After 7 days of subcutaneous injection,8 rats were randomly selected to collect samples for modeling and vitamin D testing.The successful modeling was confirmed,and then the rats were fed in groups.The low selenium group was given inorganic selenium[1.0mg/(kg·w)sodium selenite]by gavage,the high selenium group was given inorganic selenium[5.0mg/(kg·w)sodium selenite]by gavage,and the non selenium group was also given an equal amount of physiological saline by gavage.After 4 weeks of continuous collection,samples were collected to test serum bone metabolism biochemical indicators;Administer a one-time intramuscular injection of 9000 U/100g of vitamin D,continue the above dose of selenium gavage for 4 weeks,and collect samples to test serum bone metabolism biochemical indicators.Results When supplementing with selenium alone,there was no significant increase in serum 25 hydroxyvitamin D levels in the experimental group rats,and there was no significant difference between the groups.However,the alkaline phosphatase in the high selenium group was lower than that in the non supplemented and low selenium groups(P<0.05);After selenium combined with vitamin D supplementation treatment,the serum levels of 25 hydroxyvitamin D in the experimental group rats were significantly increased compared to before,and the low selenium group was higher than the non selenium group(P<0.05).Conclusion Low selenium can promote the conversion of vitamin D to 25 hydroxyvitamin D in a rat model of metabolic bone disease.关键词
硒/维生素D/代谢性骨病Key words
Selenium/Vitamin D/Metabolic Bone Disease分类
特种医学引用本文复制引用
赵兴娇,朱泽宇,张永芳..无机硒对大鼠代谢性骨病的影响[J].哈尔滨医药,2025,45(5):1-5,5.基金项目
湖北省卫健委科研项目(WJ2021F094) （WJ2021F094）
