黑龙江动物繁殖2025，Vol.33Issue(5)：1-9,9.DOI:10.19848/j.cnki.ISSN1005-2739.2025.10.0001
湖羊SRD5A2基因多态性与繁殖性状的关联性分析
Genetic polymorphism of SRD5A2 gene and its associations with reproductive traits in Hu sheep
摘要
Abstract
The expression of steroid 5-alpha reductase type 2(SRD5A2)in animal reproductive system is closely correlated with germ cell maturation and embryo survival.To investigate the impact of SRD5A2 gene variations on the reproductive performance of Hu sheep,ear tissues were collected from 384 individuals for DNA extraction.Pooled PCR and Mass ARRAY genotyping were applied to screen the single nucleotide polymorphisms(SNPs)in SRD5A2 gene,assess its genetic diversity,and analyze the association with litter size and lambing interval.Results showed that four SNPs were found within the 3'UTR of SRD5A2 gene:g.46 935 A>G,g.48 348 A>G,g.48 388 A>T,and g.48 746 A>G,in each of 4 loci three genotypes were detected,respectively,and in Hardy-Weinberg equilibrium.At g.48 388 A>T locus,AA genotypes had a significantly higher litter size from parities 1-2,and 1-3 than AT genotypes.At g.48 746 A>G locus,AG genotypes produced a significantly higher litter size in the 4th parity than GG genotypes and AA genotypes exhibited a significantly shorter lambing interval between parities 2-3 than GG genotypes.Moreover,4 haplotypes and 5 diplotypes were detected in 4 SNPs,and individuals with diplotypes H3H4(GGAATTAG)and H4H4(GGAATTGG)exhibited higher litter sizes.In conclusion,the g.48 388 A>T and g.48 746 A>G loci in SRD5A2 gene are associated with the reproductive traits and could be the molecular markers in the marker-assisted selection in Hu sheep.关键词
湖羊/类固醇5α还原酶Ⅱ型(SRD5A2)/单核苷酸多态性(SNP)/产羔数/产羔间隔Key words
Hu sheep/SRD5A2/SNP/litter size/lambing interval分类
农业科技引用本文复制引用
仓振,刘馨,孟旭铭,谢婧,茆达干..湖羊SRD5A2基因多态性与繁殖性状的关联性分析[J].黑龙江动物繁殖,2025,33(5):1-9,9.基金项目
国家重点研发计划项目(2022YFD1300205) （2022YFD1300205）
