黑龙江动物繁殖2025，Vol.33Issue(5)：20-25,6.DOI:10.19848/j.cnki.ISSN1005-2739.2025.10.0003
家畜精液冷冻保存技术研究
Research on cryopreservation technology of livestock semen
摘要
Abstract
Semen cryopreservation technology is a key technique for preserving excellent male livestock germplasm resources and promoting the development of modern animal husbandry.This review systematically summarizes the research progress of this technology:it outlines the mechanisms of damage caused by freezing to sperm motility,plasma membrane and acrosome structure,and fertilizing ability,and emphasizes the central role played by oxidative stress;It compares the principles and application limitations of mainstream methods such as slow freezing,vitrification and freeze-drying.The research progress on key additives in semen extenders is discussed,including the roles of various antioxidants(enzymes,vitamins,plant extracts and nanomaterials),sugars,buffers and cryoprotectants(glycerol,egg yolk,novel antifreeze proteins)in improving post-thaw sperm quality.Although this technology has become increasingly mature,effectively inhibiting ice crystal formation,precisely regulating oxidative stress,and optimizing individualized freezing protocols remain critical challenges to be breakthrough in future research.关键词
精液品质/冷冻方法/氧化应激/冷冻程序/冷冻保护剂Key words
semen quality/freezing methods/oxidative stress/freezing protocol/cryoprotectant分类
农业科技引用本文复制引用
赵程禹,杜鹏举,高琴,王大祥,陆宏辉,张艳丽,王子玉..家畜精液冷冻保存技术研究[J].黑龙江动物繁殖,2025,33(5):20-25,6.基金项目
江苏省种业揭榜挂帅项目(JBGS[2021]113) （JBGS[2021]113）
