黑龙江动物繁殖2025，Vol.33Issue(5)：45-50,6.DOI:10.19848/j.cnki.ISSN1005-2739.2025.10.0007
基于Smart-seq2挖掘猪早期胚胎pDUXC类基因及其潜在功能分析
Identification and functional characterization of DUX-like genes in early porcine embryos using Smart-seq2
摘要
Abstract
This study aimed to investigate DUX family genes in the early development of porcine embryos and their potential functions.Using Smart-seq2 single-embryo transcriptome sequencing combined with StringTie assembly,we performed a systematic analysis of 4-cell stage in vitro fertilized porcine embryos and successfully identified a DUX family gene,designated pDUXC,located at the terminal region of chromosome 17.Bioinformatic analyses suggested that the pDUXC protein contain two conserved HOX homeodomains and a proline-rich transcriptional activation region,along with distinct nuclear localization signals and the features of a transcription factor.Phylogenetic analysis revealed that pDUXC clusters closely with human DUX4,suggesting a high degree of evolutionary conservation between two species.Taken together,these findings suggest that pDUXC may participate in zygotic genome activation(ZGA)by activating ERVs,thereby driving the transcription of genes essential for early development.关键词
猪/pDUXC类基因/胚胎/转录本组装/Smart-seq2Key words
porcine/DUX-Like genes/embryos/transcriptome assembly/Smart-seq2分类
生物科学引用本文复制引用
付博,马红,汪亮,郭镇华,王芳,霍秀鹏,刘娣..基于Smart-seq2挖掘猪早期胚胎pDUXC类基因及其潜在功能分析[J].黑龙江动物繁殖,2025,33(5):45-50,6.基金项目
黑龙江省省属科研院所科研业务费项目(CZKYF2024-1-B006) （CZKYF2024-1-B006）
