湖泊科学2025，Vol.37Issue(6)：1918-1925,8.DOI:10.18307/2025.0613
水环境中甲基膦酸含量变化及其对"藻—磷"关系响应的探究
Exploration of methylphosphonate content variations in aquatic environments and their re-sponse to the algae-phosphorus relationship
摘要
Abstract
Methylphosphonate(MPn),a typical organophosphonate characterized by a C-P bond,profoundly influences phos-phorus cycling and methane(CH4)production mechanisms in aquatic ecosystems through its biosynthesis and degradation proces-ses.However,there is limited research on the dynamics of MPn in water bodies and the MPn accumulation capacity of algae.This study employed liquid chromatography-tandem quadrupole time-of-flight mass spectrometry(LC-MS/MS)to quantify MPn in 21 water samples and 15 algal species.Combined with field monitoring,algal laboratory cultivation,and raw water incubation experi-ments(including MPn/Pi addition,BES treatment,algal filtration,and dark treatment),the relationship between MPn dynamics and CH4 generation was investigated.The results revealed that MPn was detected in 52.4％(11/21)of water samples((1.50±0.24)-(6.99±0.59)μg/L),and 93.3％(14/15)of algal strains accumulated intracellular MPn((1.87±0.57)-(22.24±5.81)μg/L).Notably,Microcystis sp.FACHB-3602 exhibited dynamic MPn accumulation during 7 days of cultivation(peak value:(8.63±0.85)μg/L),indicating that algae are a significant biological source of MPn in aquatic ecosystems.In both water samples and algae,the contribution of MPn-P to dissolved organic phosphorus(0.70％-37.85％,0.21％—0.90％)was signifi-cantly higher than that of MPn-C to dissolved organic carbon(0.00％—0.05％,0.00％—0.01％),highlighting the dominant role of MPn in phosphorus cycling from an ecological stoichiometric perspective.Raw water incubation experiments demonstrated that MPn addition increased CH4 production by 157.43％compared to the control,while the simultaneous addition of inorganic phos-phorus(Pi)suppressed CH4 generation.Algal filtration reduced CH4 production by 23.96％,whereas dark treatment promoted CH4 accumulation.These findings suggest that algal-bacterial interactions regulate MPn turnover and aerobic CH4 production,modulated by inorganic phosphorus availability.This study provides critical theoretical insights for further exploration of MPn's role in aquatic phosphorus cycling and aerobic CH4 production mechanisms.关键词
甲基膦酸/藻类/有氧产甲烷/磷循环Key words
Methylphosphonate/algae/aerobic methane production/phosphorus cycling引用本文复制引用
李清,袁翰卿,李哲,肖艳,高镱萌,邬小静..水环境中甲基膦酸含量变化及其对"藻—磷"关系响应的探究[J].湖泊科学,2025,37(6):1918-1925,8.基金项目
国家自然科学基金项目(52470202,U2340222)联合资助. （52470202,U2340222）
