湖泊科学2025，Vol.37Issue(6)：2118-2131,14.DOI:10.18307/2025.0651
太湖沉积物腐殖酸结构特征及其对产甲烷的影响
Structural characteristics of humic acid in sediments of Lake Taihu and its influence on methanogenesis
摘要
Abstract
Humic acid inhibits methane production by competing with the methanogenic process for electrons and is a natural strate-gy for reducing greenhouse gas emissions from lakes.There is still a lack of systematic understanding of the structural characteris-tics of humic acid in different lake habitat types and its impact on the methanogenesis process.This study aimed to systematically analyze the structural characteristics of humic acid(HA)in sediments from different regions of Lake Taihu and its effects on meth-anogenesis.The sampling area covers East Taihu Lake,Meiliang Bay,the central lake area and Dapukou,and surface sediments were collected for quantitative analysis.The content,structural characteristics and redox properties of HA(including electron ac-cepting capacity(EAC)and electron donating capacity(EDC))were characterized by Fourier transform infrared spectroscopy,spectrophotometry,elemental analysis,electron transfer capacity measurement and quantitative measurement of quinone groups.The results showed significant spatial differences in HA content and its redox properties among the four regions of Lake Taihu.The HA content in sediments from grass-type lakes(such as East Lake Taihu)was the highest,while the EAC of HA in sediments from algae-type lakes was the strongest.Correlation analysis showed that the EAC of HA was significantly negatively correlated with the carbon/nitrogen ratio of sediments and significantly positively correlated with the aromatic condensation degree of HA.To further clarify the effect of Lake Taihu HA on the methanogenesis process,this study explored the effect of HA from different EAC on the methanogenesis process in a methanogen enrichment system and monitored the methanogenesis process by measuring the methane accumulation and the relative activity of coenzyme F420.The results showed that as the EAC of HA increased,the inhibitory effect of HA on the methanogenesis process became more significant and the relative activity of coenzyme F420 became lower.This finding suggests that EAC from lake HA inhibits methane production by suppressing the activity of methanogens.Future studies should fur-ther focus on the effects of different matrices on the formation mechanism of lake HA and the key factors affecting the EAC of HA to fully understand the potential role of lake HA in climate change regulation.关键词
腐殖酸/电子接受能力/产甲烷/太湖/藻型湖区/草型湖区Key words
Humic acid/electron accepting capacity/methane production/Lake Taihu/algal-dominated regions/macrophyte-dominated regions引用本文复制引用
周文杰,王洪伟,赵成,荀凡,韩雪欣,许妍,邢鹏..太湖沉积物腐殖酸结构特征及其对产甲烷的影响[J].湖泊科学,2025,37(6):2118-2131,14.基金项目
江苏省自然科学基金项目(BK20220015)和国家自然科学基金项目(92251304)联合资助. （BK20220015）
