红外与毫米波学报2025，Vol.44Issue(5)：631-640,10.DOI:10.11972/j.issn.1001-9014.2025.05.001
近红外二区量子点标记外泌体治疗伴缺血周围神经损伤过程的体内成像研究
NIR-Ⅱ quantum dot-labeled exosomes'imaging in treatment of ischemic peripheral nerve injury
摘要
Abstract
Ischemia is a significant factor affecting the repair of peripheral nerve injuries,while exosomes have been shown to promote angiogenesis.To further investigate the detailed processes and efficacy of exosome thera-py for ischemic peripheral nerve injuries,this study utilized glucose-modified near-infrared-II(NIR-Ⅱ)quantum dots(QDs)to label adipose-derived stem cell exosomes(QDs-ADSC-Exos),enabling long-term in vivo NIR-Ⅱ imaging of exosome treatment for ischemic peripheral nerve damage.Experimental results confirmed that QDs can be used for non-invasive in vitro labeling of exosomes,with QDs-ADSC-Exos exhibiting strong fluorescence signals in the NIR-Ⅱ window and demonstrating favorable NIR-Ⅱ imaging characteristics in vivo.Notably,QDs-ADSC-Exos showed accumulation at the site of nerve injury in cases of ischemic peripheral nerve damage.Func-tional neurological assessments indicated that QDs-ADSC-Exos effectively promoted neural regeneration.This study highlights the potential of exosomes in treating ischemic peripheral nerve injuries and elucidates the spatio-temporal characteristics of exosome therapy,providing objective evidence for the further optimization of exosome-based treatment protocols.关键词
近红外二区成像/量子点/外泌体/周围神经损伤/血管损伤Key words
NIR-Ⅱ imaging/quantum dots/exosomes/peripheral nerve injury/vascular injury分类
基础医学引用本文复制引用
小仓心吾,邵珂萌,张晓,李惠珠,冯思嘉,王月明,陈俊,吴德华,沃雁..近红外二区量子点标记外泌体治疗伴缺血周围神经损伤过程的体内成像研究[J].红外与毫米波学报,2025,44(5):631-640,10.基金项目
Supported by the National Natural Science Foundation of China(82371373,W2412120) （82371373,W2412120）
the Shanghai Natural Science Foundation(21ZR1436100). （21ZR1436100）
