红外与毫米波学报2025，Vol.44Issue(5)：654-662,9.DOI:10.11972/j.issn.1001-9014.2025.05.003
基于临床可用荧光探针的NIR-Ⅱ优质亚窗口的活体成像研究
In vivo fluorescene imaging in the superior NIR-Ⅱ sub-window using clinical-grade fluorescent dye
摘要
Abstract
NIR-Ⅱ fluorescence imaging demonstrates significant advantages in biological imaging with its high signal-to-background ratio(SBR)and deep tissue penetration,showing broad application prospects in biomedical fields.The clas-sification of NIR-Ⅱ imaging windowsfacilitates the optimization of imaging processes.Among these,the 1 400-1 500 nm imaging window benefits from its unique water absorption characteristics,enabling effective suppression of scattering background and achieving high-contrast imaging.This study systematically evaluates the imaging potential of the 1 400-1 500 nm window through simulation studies and in vivo experiments.To advance the clinical translation of fluores-cence imaging in the 1400-1500 nm window,indocyanine green(ICG),an organic small-molecule dye approved by the U.S.Food and Drug Administration(FDA),was employed as the fluorescent probe.Utilizing its extended fluores-cence emission tail in the NIR-Ⅱ region,high-contrast and high-resolution imaging of mouse vasculature and intestinal structures was achieved in the 1 400-1 500 nm window.Furthermore,in combination with methylene blue(MB),an-other FDA-approved agent,high-quality dual-channel NIR-Ⅱ imaging was successfully implemented enabling precise lo-calization of blood vessels and lymph nodes in mice.This research further explores the unique advantages of the 1 400-1 500 nm imaging window in biological imaging and its clinical application potential.It also provides valuable referenc-es for the clinical translation of NIR-Ⅱ fluorescence imaging.关键词
荧光成像/NIR-Ⅱx/近红外二区/双通道成像/临床手术导航Key words
fluorescence imaging/NIR-Ⅱx/NIR-Ⅱ/dual-channel imaging/clinical surgical navigation分类
物理学引用本文复制引用
李佳一,张沛锦,夏启铭,钱骏..基于临床可用荧光探针的NIR-Ⅱ优质亚窗口的活体成像研究[J].红外与毫米波学报,2025,44(5):654-662,9.基金项目
the National Key R&D Program of China(2022YFB3206000) （2022YFB3206000）
the National Natural Science Foundation of China(U23A20487) （U23A20487）
Dr.Li Dak Sum&Yip Yio Chin Development Fund for Regenerative Medicine,Zhejiang University （）
the National Natural Science Foundation of China(61975172). （61975172）
