家畜生态学报2025，Vol.46Issue(10)：28-35,88,9.DOI:10.3969/j.issn.1673-1182.2025.10.005
和杂牛脂肪沉积相关功能基因及分子标记筛选的研究
Studies on Functional Genes and Molecular Markers Screening Related to Fat Deposition in Wagyu-Holstein Cross Cattle
摘要
Abstract
Intramuscular fat deposition plays an important role in the quality and flavor of beef.Explo-ring the molecular markers related to meat quality is of great theoretical significance for the genetic im-provement of beef cattle.In this study,333 Wagyu-Holstein cross cattle(hereinafter referred to as mixed cattle)from three populations in Shandong were selected as the research objects.SNP loci were detected by GBS Reduced-Representation Sequencing technology,and the 6-7 intercostal intramuscular fat ratings were collected.Genome-wide association study(GWAS)was conducted based on mixed linear model.Significant single nucleotide polymorphisms(SNPs)were annotated with published ATAC-seq data.Finally,MYT1L,DOCK 5,UACA and SLC25A34 genes were identified as the key genes affecting the quality grade of mixed beef.rs382972390 and Chr8:111709102 were candidate markers related to meat quality grade,which could be used for marker-assisted selection of meat quality traits in mixed cattle populations.关键词
简化基因组测序/脂肪沉积/全基因组关联分析/SNP位点Key words
reduced-representation sequencing/fat deposition/GWAS/SNP loci分类
农业科技引用本文复制引用
殷艳平,成海建,宋恩亮,姜富贵,胡鑫,游伟,谢良佳,胡洪杰,张德敏,刘育含..和杂牛脂肪沉积相关功能基因及分子标记筛选的研究[J].家畜生态学报,2025,46(10):28-35,88,9.基金项目
国家现代农业(肉牛牦牛)产业技术体系专项(CARS-37) （肉牛牦牛）
山东省牛产业技术体系项目(SDAIT-09-03) （SDAIT-09-03）
济南市农业应用技术创新计划项目(CX202111) （CX202111）
山东省农业重大技术协同推广计划项目(SDNYXTTG-2023-27) （SDNYXTTG-2023-27）
泰山产业领军人才工程专项经费 （）
