家畜生态学报2025，Vol.46Issue(10)：36-43,8.DOI:10.3969/j.issn.1673-1182.2025.10.006
Let-7b-5p靶向MSI2和FOXC1调控绒山羊毛囊干细胞的增殖和分化
Let-7b-5p Targets MSI2 and FOXC1 to Regulate the Proliferation and Differentiation of HFSCs in Cashmere Goat
摘要
Abstract
The objective of this study was to explore the mechanism of miRNA let-7b-5p on the prolif-eration and differentiation of hair follicle stem cells(HFSCs)in cashmere goat.HFSCs of cashmere goats were isolated and identified,let-7b-5p and NC mimics were transfected into cashmere goat HFSCs by Lipo-fectamine® 3000.The effects of let-7b-5p on the cell proliferation were detected by CCK8 and EdU experi-ments.The proliferation genes(KI67,PCNA,AKT and m TOR)and the differentiation genes(K6,S100A3,Notch1 and β-catenin)were detected to identify their expression variation.A dual luciferase re-porter vector was also constructed to validate the relationship between let-7b-5p and its predicted target genes.The results indicated that let-7b-5p significantly inhibited the proliferation ability of HFSCs(P＜0.01),and the expression of key cell proliferation marker genes PCNA and KI67 as well as AKT signaling pathway-related genes in HFSCs treated with let-7b-5p significantly decreased(P＜0.01).Let-7b-5p sig-nificantly activated the expression of hair follicle differentiation-related marker genes K6 and S100A3 and Notch1 pathway(P＜0.01).When let-7b-5p was overexpressed,the luciferase reporter activity of MSI2 and FOXC1 genes significantly decreased.In summary,let-7b-5p directly targets FOXC1 and MSI2 genes,inhibiting the proliferation of Cashmere goat HFSCs via the AKT signaling pathway;meanwhile,let-7b-5p promotes the directed differentiation of HFSCs into hair follicle cells through the Notch signaling pathway.关键词
绒山羊/毛囊干细胞/增殖/let-7b-5pKey words
cashmere/hair follicle stem cells/proliferation/let-7b-5p分类
畜牧业引用本文复制引用
闫海龙,李艳,金妙函,高晔,李进霞,孙文慧,康佳琪,王小龙,陈玉林..Let-7b-5p靶向MSI2和FOXC1调控绒山羊毛囊干细胞的增殖和分化[J].家畜生态学报,2025,46(10):36-43,8.基金项目
山西省科技厅自然科学研究面上项目(202203021221208) （202203021221208）
国家自然科学基金(32072719、31972526) （32072719、31972526）
山西省卫健委科技兴医创新项目(2023XM033) （2023XM033）
大同市科技局项目(2024048) （2024048）
