家畜生态学报2025，Vol.46Issue(10)：81-88,8.DOI:10.3969/j.issn.1673-1182.2025.10.012
鸡白痢沙门菌sRNA的预测及毒力相关sRNA的筛选
Prediction of Non-Coding sRNAs and Screening of Virulence-Related sRNAs of Salmonella pullorum
摘要
Abstract
To predict the non-coding sRNAs present in Salmonella pullorum and to screen the sRNAs related to bacterial survival and pathogenicity,the experiment firstly sequenced Salmonella pullorum by using small RNA-seq(sRNA-seq)technology,selected representative sRNAs for bioinformatics analysis and their target genes prediction,and then validated the candidate sRNAs by RT-PCR.Finally,the candi-date sRNA transcript levels were detected in the chicken macrophage HD11 infection model.The results showed 111 newly predicted sRNAs from sRNA-seq,among which 7 sRNAs with the highest Reads num-ber were screened out.Secondary structure prediction showed that all 7 sRNAs exhibited typical stem-loop structures.KEGG and GO enrichment analysis of target genes suggested that the candidate sRNAs were involved in several physiological processes,such as transmembrane transport and bacterial metabolism.RT-PCR results showed that five of the candidate sRNAs were detected at the transcriptional level.Additionally,results from the cellular infection model indicated that sRNA12 is most likely involved in the bacterial infection and patho-genic process.This study identified new sRNAs in Salmonella pullorum and highlights those potentially involved in bacterial survival and pathogenicity,providing a scientific basis for an in-depth investigation into the patho-genic mechanism and potential inhibitory targets of Salmonella pullorum.关键词
鸡白痢沙门菌/非编码小RNA/靶基因预测/胞内生存Key words
Salmonella pullorum/sRNA/target gene prediction/intracellular survival分类
畜牧业引用本文复制引用
何婷,冉旭华,丁永辉,孙羽伶,李天森..鸡白痢沙门菌sRNA的预测及毒力相关sRNA的筛选[J].家畜生态学报,2025,46(10):81-88,8.基金项目
海南省自然科学基金高层次人才项目(823RC468) （823RC468）
国家自然科学地区基金项目(32360878) （32360878）
海南省自然科学基金(321RC1020) （321RC1020）
海南省研究生创新科研课题(Qhys2023-229) （Qhys2023-229）
