基础医学与临床2025，Vol.45Issue(11)：1409-1414,6.DOI:10.16352/j.issn.1001-6325.2025.11.1409
下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移
Downregulation of Talin1 inhibits migration of pulmonary arterial smooth muscle cells(PASMCs)induced by serum of rat models with HPS
摘要
Abstract
Objective To evaluate the role of Talin1 in the migration of rat pulmonary artery smooth muscle cells(PASMCs)induced by the serum from rats with hepatopulmonary syndrome(HPS).Methods Twenty male Spra-gue-Dawley(SD)rats were used as HPS rat models by chronic common bile duct ligation,the serum was collected from abdominal aorta.PASMCs were seeded in 6-well and 24-well plates and randomly divided into control group and HPS group.The cells were transfected with Talin1 or control siRNA.The normal rat serum or HPS rat serum with a final concentration of 5％was added respectively.At 24 hours after cell transfection or at 24 hours(T1),48 hours(T2)and 72 hours(T3)of cell incubation,the protein expression of Talin1 and active Integin β1 in PASMCs were determined by Western blot;The migration of PASMCs was measured by Transwell chamber(T1)and scratch assay(T1 to T3).Results Compared to control group,with the extension of the stimula-tion time in the HPS group,the expression of Talin1 protein was upregulated,and the migration of PASMCs was gradually enhanced(P＜0.05);Talin1 siRNA effectively silenced the Talin1 gene;The expression of ac-tive Integin β1 protein and the migration of PASMCs in the HPS group+si control were enhanced(P＜0.05);Compared with the HPS group+si control,the expression of active Integin β1 protein and the migration of PASMCs in the HPS group+siTalin1 were significantly inhibited(P＜0.05).Conclusions Downregulating ex-pression of Talin1 protein inhibits migration of PASMCs and expression of active Integin β1 protein induced by the serum from HPS rats.关键词
肝肺综合征/肺动脉平滑肌细胞/踝蛋白1/整合素β1Key words
hepatopulmonary syndrome/pulmonary arterial smooth muscle cells/Talin 1/Integrin β1分类
临床医学引用本文复制引用
陈杨,文静,石岚,杨勇,易斌,陈林..下调Talin1抑制肝肺综合征模型大鼠血清诱导的PASMCs迁移[J].基础医学与临床,2025,45(11):1409-1414,6.基金项目
国家自然科学基金(82270656) （82270656）
重庆市自然科学基金(CSTB2022NSCQ-MSX0218) （CSTB2022NSCQ-MSX0218）
重庆市教委科技研究计划青年项目(KJQN202401137) （KJQN202401137）
