基础医学与临床2025，Vol.45Issue(11)：1444-1450,7.DOI:10.16352/j.issn.1001-6325.2025.11.1444
IL-37抑制巨噬细胞介导的浆细胞性乳腺炎
IL-37 inhibits macrophage-mediated plasma cell mastitis
摘要
Abstract
Objective To investigate whether interleukin-37(IL-37)affects macrophage M1 polarization via nu-cleotide oligomerization of structural domain receptor protein 1(NOD1)/nuclear factor κB(NF-κB)in plasma cell mastitis.Methods A total of 15 patients with plasma cell mastitis were recruited according to the collection standard as inflammatory breast tissue and normal breast tissue with a distance of ≥ 3 cm from the edge of the in-flammatory breast tissue.QPCR was performed to detect NOD1 and IL-3.Phorbol ester(PE)was used to induce THP-1 cells to differentiate into resting macrophages(M0).Lipopolysaccharide(LPS)combined with interferon(IFN-γ)was used to induce the polarization of M0 macrophages towards the M1 phenotype.NOD1 lentiviral RNA interference or over-expression vectors were constructed to regulate the expression of NOD1 in M1 macrophages.M0 macrophages were pretreated with IL-37 recombinant protein and then incubated with LPS and IFN-γ for induction.The expression of NOD1,IL-37,M1 markers(IL-6 and iNOS)and M2 markers(IL-10 and Arg-1)was quantified by qPCR.Western blot was employed to assess the protein level of NOD1,NF-κB p65,and p-NF-κB p65.Co-immunoprecipitation was used to detect the interaction between NOD1 and IL-37.Results Up-regulation of NOD1 and down-regulation of IL-37 as were found in inflammatory breast tissues(P＜0.05).Compared to M0 cells,M1 cells showed up-regulated NOD1 and M1 markers and the elevated phosphorylated NF-κB p65 but the down-regulated IL-37(P＜0.05).In M1 macrophages,both NF-κB inhibitor and NOD1 knockdown led to the down-regulation of NOD1 and M1 markers and caused a decrease in the phosphorylated NF-κB p65(P＜0.05).IL-37 recombinant protein decreased phosphorylation of NOD1,M1 marker and NF-κB p65,which was reversed by over-expression of NOD1.IL-37 may interact with NOD1(P＜0.05).Conclusions IL-37 may inhibit M1 polarization in macrophages by down-regulating NOD1/NF-κB pathway thereby preventing plasma cell mastitis progression.关键词
浆细胞性乳腺炎/巨噬细胞/M1极化/白介素-37/核苷酸寡聚化结构域受体蛋白1Key words
plasma cell mastitis/macrophage/M1 polarization/interleukin-37/nucleotide oligomerization structural domain receptor protein 1分类
临床医学引用本文复制引用
邓友缘,赵红军,叶丽芬,李靖勇,张怀孝,张超,王建国..IL-37抑制巨噬细胞介导的浆细胞性乳腺炎[J].基础医学与临床,2025,45(11):1444-1450,7.基金项目
湖南省卫生健康委科研计划(D202304018543) （D202304018543）
