基础医学与临床2025，Vol.45Issue(11)：1457-1462,6.DOI:10.16352/j.issn.1001-6325.2025.11.1457
miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡
miR-374c-5p reduces hydrogen peroxide induced apoptosis of human umbilical vein endothelial cells
摘要
Abstract
Objective To explore the protective effect of miR-374c-5p on hydrogen peroxide(H2O2)-induced apoptosis of human umbilical vein endothelial cells(HUVECs).Methods HUVECs were cultured in vitro and the harvested cells were divided into four groups:control group,H2O2 group,H2O2+miR-374c-5p mimics trans-fection group,and H2O2+miR-374c-5p inhibitor transfection group.Cell activity was assessed by CCK-8 prolifer-ation rate assay,apoptosis was detected by TUNEL staining microscopy.Expression of miR-374c-5p and Fas mRNA by RT-qPCR,and Fas protein in HUVECs by was detected by Western blot.Results Proliferation of HUVECs was significantly inhibited(P＜0.001);H2O2 was significantly increased as compared with the H2O2 in-tervention group(P＜0.001);Proliferation in H2O2+miR-374c-5p inhibitor transfection group was significantly increased as compared to H2O2 intervention group(P＜0.001).Conclusions miR-374c-5p protectes the HUVECs against apoptosis induced by H2O2.关键词
miR-374c-5p/人脐静脉内皮细胞(HUVECs)/过氧化氢/氧化应激/凋亡Key words
miR-374c-5p/human umbilical vein endothelial cells(HUVECs)/hydrogen peroxide(H2O2)/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
甲宗虎,晋群,韩淑芳,胡毓洪,任昌振,李云萍,刘文燕..miR-374c-5p降低过氧化氢诱导的人脐静脉内皮细胞凋亡[J].基础医学与临床,2025,45(11):1457-1462,6.基金项目
潍坊医学院附属医院科技发展项目(2023FYZ013) （2023FYZ013）
