摘要
Abstract
Objective To investigate the therapeutic effects and mechanisms of Alisma orientale extract(zexie)on gestational diabetes mellitus(GDM)rats by regulating the miR-4417/TGF-β pathway.Methods GDM model rats were randomly divided into a diabetic group,low,medium,high-dose zexie group,insulin group,miR-NC group,miR-4417 mimic group,miR-4417 inhibitor group,and zexie+miR-4417 mimic+LY364947 group,with 12 rats in each group.Additionally,12 normal pregnant rats served as the control group.Hematoxylin and eosin(HE)staining was used to evaluate pancreatic islet pathology.RT-qPCR and Western blot were employed to measure the mRNA and protein levels of various factors.Results Compared with the diabetic group,the glucose,lipid,oxida-tive stress indicators,and pancreatic islet pathological damage were significantly improved(P＜0.05)in the low,medium and high-dose zexie groups and the insulin group.The expression levels of miR-4417 and TGF-β mRNA were notably reduced(P＜0.05),with the most significant changes observed in the high-dose zexie group(P＜0.05).Compared with the miR-NC group,the TGF-β protein level was significantly increased(P＜0.05)in the miR-4417 mimic group and decreased(P＜0.05)in the miR-4417 inhibitor group.Compared with the diabetic group,the oxidative stress and reactive oxygen species(ROS)accumulation were significantly lower(P＜0.05)in the zexie,zexie+miR-4417 mimic,and zexie+miR-4417 mimic+LY364947 groups.Compared with the miR-4417 mimic group,the oxidative stress and ROS accumulation were significantly reduced(P＜0.05)in the zexie+miR-4417 mimic group.No significant differences were found between the zexie group and the zexie+miR-4417 mimic+LY364947 group.Conclusions Zexie can improve glucose and lipid levels,reduce insulin resistance,and control oxidative stress and damage in GDM rats,possibly through the regulation of the miR-4417/TGF-β pathway.关键词
泽泻/妊娠期糖尿病/TGF-β通路/miR-4417Key words
Alisma orientalis/gestational diabetes/TGF-β pathway/miR-4417分类
临床医学
