激光生物学报2025，Vol.34Issue(5)：409-416,8.DOI:10.3969/j.issn.1007-7146.2025.05.004
circHIPK3通过竞争性结合miR-599调控CD276表达促进肝癌发展
circHIPK3 Promotes Hepatocellular Carcinoma Development by Competitively Binding miR-599 to Regulate CD276 Expression
摘要
Abstract
Hepatocellular carcinoma(HCC)is the third leading cause of cancer-related mortality worldwide,characterized by poor prognosis and a high recurrence rate.To investigate the regulatory mechanism of circHIPK3 in HCC,we analyzed the expression levels of HIPK3 and its circular RNA isoform,circHIPK3,using quantitative real-time PCR(qRT-PCR),and found both to be significantly upregulated in HCC cells.Dual-luciferase reporter assays confirmed that circHIPK3 directly binds to miR-599,and that miR-599 targets the 3'untranslated region(UTR)of CD276.Furthermore,we established a stable HCC cell line with circHIPK3 knockdown(si-circHIPK3)and transfected miR-599 mimics to assess functional consequences.The results demonstrated that circHIPK3 can act as a competing endogenous RNA(ceRNA)to sponge miR-599,thereby alleviating its inhibitory effect on CD276 and upregulating CD276 expression,and enhancing the proliferation and migration ability of HCC cells.Collectively,our findings elucidate the circHIPK3/miR-599/CD276 regulatory axis in HCC progression,providing novel molecular insights into the malignant behavior of HCC cells and highlighting a potential therapeutic target.关键词
肝癌/环状同源域相互作用蛋白激酶/微小核糖核酸-599/分化簇276/治疗靶点Key words
hepatocellular carcinoma/circHIPK3/miR-599/CD276/therapeutic target分类
生物学引用本文复制引用
戴一岚,欧阳密,李志伟,周号悦,蒋志翔,丁小凤,李立民..circHIPK3通过竞争性结合miR-599调控CD276表达促进肝癌发展[J].激光生物学报,2025,34(5):409-416,8.基金项目
国家自然科学基金项目(81872256) （81872256）
湖南师范大学交叉学院培育团队项目(2023JC203). （2023JC203）
