激光生物学报2025，Vol.34Issue(5)：442-450,9.DOI:10.3969/j.issn.1007-7146.2025.05.007
牛蒡子苷元对庆大霉素诱导的大鼠急性肾损伤的治疗作用和机制研究
Research on the Therapeutic Effects and Mechanisms of Arctigenin in Treating Gentamicin-induced Acute Kidney Injury in Rats
摘要
Abstract
To investigate the therapeutic effect and mechanism of arctigenin(ATG)on gentamicin(GM)induced acute kidney injury(AKI)in rats via activating the nuclear factor erythroid 2-related factor 2(Nrf2)/heme oxygenase-1(HO-1)signaling pathway and inhibiting the NF-κB signaling pathway,36 male SD rats were selected and randomly divided into a control group(6 rats)and a model establishment group(30 rats).The model group received intraperitoneal injection of 100 mg/kg GM for 7 consecutive days to establish the AKI model.The successfully modeled rats were then randomly assigned to the model group,positive control group(5 mg/kg benazepril hydrochloride),and ATG low-,medium-,and high-dose groups(1,3,9 mg/kg ATG),with 6 rats in each group.The following parameters were measured:serum urea nitrogen(BUN)and creatinine(SCR)levels;renal tissue levels of superoxide dismutase(SOD),reduced glutathione(GSH),catalase(CAT),malondialdehyde(MDA),interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and kidney injury molecule-1(KIM-1),as well as the mRNA and protein expression levels of Nrf2,HO-1,and nuclear factor-kappa B p65(NF-κB p65).Compared with the model group,ATG treatment significantly reduced serum levels of BUN and SCR in rats(P<0.01).In renal tissues,it markedly upregulated the mRNA and protein expression of antioxidant indicators(SOD,CAT,GSH)and key factors of the Nrf2/HO-1 pathway(Nrf2,HO-1)(P<0.05 or P<0.01),while downregulating the expression of oxidative damage product(MDA),inflammatory factors(IL-1β,IL-6,TNF-α),kidney injury marker(KIM-1),as well as NF-κB p65 mRNA and protein(P<0.05 or P<0.01).Histopathological examination revealed that ATG treatment improved renal tubular epithelial cell morphology,attenuated tubular atrophy,fibrosis,and inflammatory infiltration.These results demonstrate that ATG exerts a multi-target therapeutic effect on GM-induced AKI by synergistically activating the Nrf2/HO-1 pathway to enhance antioxidant capacity and inhibiting the NF-κB pathway to alleviate inflammatory response,thereby providing a theoretical basis for its clinical translation.关键词
牛蒡子苷元/庆大霉素/急性肾损伤/核因子E2相关因子2/血红素加氧酶1信号通路/核因子κB信号通路Key words
actigenin/gentamicin/acute kidney injury/nuclear factor erythroid 2-related factor 2/heme oxygenase-1 signaling pathway/nuclear factor-kappa B signaling pathway分类
中医学引用本文复制引用
周天意,蒋志明,陈博迪,姜炫辛,韩丽,贺气志..牛蒡子苷元对庆大霉素诱导的大鼠急性肾损伤的治疗作用和机制研究[J].激光生物学报,2025,34(5):442-450,9.基金项目
国家自然科学基金青年项目(82100040) （82100040）
湖南省教育厅科学研究项目(湘教通[2022]323号-22B0899) （湘教通[2022]323号-22B0899）
湖南省大学生创新创业训练项目(湘教通[2023]237号-4085). （湘教通[2023]237号-4085）
