激光生物学报2025，Vol.34Issue(5)：451-458,472,9.DOI:10.3969/j.issn.1007-7146.2025.05.008
下调lnc-ISG20通过调节miR-20a-5p表达对肝炎所致肝纤维化的抑制作用
Down-regulation of lnc-ISG20 Inhibits Hepatitis Induced Liver Fibrosis by Modulating miR-20a-5p Expression
摘要
Abstract
To evaluate the effect of long noncoding RNA(lncRNA)lnc-ISG20 on transforming growth factor β1(TGF-β1)-induced hepatic stellate cell activation and explore the mechanism of silencing lnc-ISG20 in anti-liver fibrosis.The GEO database was used to analyze the differential expression of lnc-ISG20 in liver fibrosis tissue and normal liver tissue.The liver fibrosis cell model was established by treating human hepatic stellate cells LX-2 with 10 ng/mL TGF-β1 and then divided into sh-NC group and sh-lnc-ISG20 group,which were transfected with sh-NC plasmid and sh-lnc-ISG20 plasmid,respectively.CCK-8 assay was used to detect the growth curve of LX-2 cells in each group.Flow cytometry was used to detect the cell cycle distribution and apoptosis rate of LX-2 cells in each group.Dual luciferase reporter assay was used to verify the targeting relationship between lnc-ISG20 and miR-20a-5p.The GEO database was used to analyze the correlation between the expression of lnc-ISG20 and miR-20a-5p in liver fibrosis tissues.qRT-PCR was used to detect the expression level of miR-20a-5p in each group of cells.Western blotting was used to detect the expression levels of p-P38,p-IκB,p-P65 and p-ASK1 proteins in the MAPK/NF-κB signaling pathway.Compared with normal liver tissue,the expression of lnc-ISG20 in liver fibrosis tissue increased(P<0.01).Compared with the sh-NC group,the proliferation activity of LX-2 cells in the sh-lnc-ISG20 group decreased(P<0.05),the cell cycle was blocked(P<0.01),and the apoptosis rate increased(P<0.01).miR-20a-5p was a target gene of lnc-ISG20(P<0.01).The expression of lnc-ISG20 is negatively correlated with miR-20a-5p in liver fibrosis tissue(P<0.01).Compared with the sh-NC group,the expression of miR-20a-5p in LX-2 cells in the sh-lnc-ISG20 group increased(P<0.01),and the expressions of p-P38,p-IκB,p-P65 and p-ASK1 proteins of the MAPK/NF-κB signaling pathway all decreased(all P<0.01).Silencing lnc-ISG20 inhibits TGF-β1-induced proliferation of LX-2 cells and promotes apoptosis by increasing miR-20a-5p expression.lnc-ISG20 is expected to become a molecular target for the treatment of liver fibrosis.关键词
肝纤维化/lnc-ISG20/miR-20a-5p/肝星状细胞/细胞凋亡Key words
liver fibrosis/lnc-ISG20/miR-20a-5p/hepatic stellate cells/cell apoptosis分类
临床医学引用本文复制引用
曾龙,高云,吴玲,刘淮,杨晶..下调lnc-ISG20通过调节miR-20a-5p表达对肝炎所致肝纤维化的抑制作用[J].激光生物学报,2025,34(5):451-458,472,9.基金项目
国家自然科学基金项目(81900560). （81900560）
