激光生物学报2025，Vol.34Issue(5)：459-465,7.DOI:10.3969/j.issn.1007-7146.2025.05.009
miR-128-3p调控SIRT1/HIF-1α通路对妊娠滋养层细胞增殖和侵袭的影响
Effects of miR-128-3p on Proliferation and Invasion of Gestational Trophoblast Cells by Modulating SIRT1/HIF-1α Pathway
摘要
Abstract
To explore the effects of microRNA-128-3p(miR-128-3p)on proliferation and invasion of gestational trophoblast cells by modulating silent information regulator 2 homolog 1(SIRT1)/hypoxia inducible factor-1α(HIF-1α)pathway,the placental tissues were collected from preeclampsia patients and normal full-term pregnant women who gave birth in the Handan Central Hospital from October 2023 to October 2024.The real-time fluorescence quantitative polymerase chain reaction experiment was performed to detect miR-128-3p in tissues and cells.JEG-3 cells were cultured and transfected with anti-miR-128-3p,si-SIRT1,and negative controls,respectively.Tetramethylthiazole blue(MTT)assay was used to detect the viability of JEG-3 cells;transwell experiment was applied to measure the migration and invasion of JEG-3 cells;Western blot was applied to measure expression of cycle-dependent protein kinase inhibitor 1A(P21),matrix metalloproteinase 2(MMP-2),E-cadherin(E-Cadherin),SIRT1,Cyclin D1(Cyclin D1),HIF-1α protein;dual luciferase assay was used to validate the targeting relationship between miR-128-3p and SIRT1.The experimental results indicate that,the expression level of miR-128-3p in placental tissues of preeclampsia group was higher than that of normal placental tissues(P<0.05).After inhibiting the expression of miR-128-3p,the expression of P21,E-cadherin and HIF-1α decreased,while the cell survival rate,the number of migrating cells and the number of invading cells,and the protein expressions of MMP-2,Cyclin D1 and SIRT1 increased(P<0.05).SIRT1 was the target gene of miR-128-3p,after simultaneously inhibiting the expression of miR-128-3p and SIRT1,the above indicators showed opposite expressions(P<0.05).Overexpression of miR-128-3p may inhibit the proliferation and invasion of gestational trophoblast cells by modulating SIRT1/HIF-1α pathway.关键词
miR-128-3p/SIRT1/缺氧诱导因子-1α/子痫前期/妊娠滋养层细胞增殖和侵袭Key words
miR-128-3p/SIRT1/hypoxia inducible factor-1α/preeclampsia/pregnancy trophoblast cell proliferation and invasion分类
临床医学引用本文复制引用
辛礼辉,田云霄,孙颖,王静,赵圆圆..miR-128-3p调控SIRT1/HIF-1α通路对妊娠滋养层细胞增殖和侵袭的影响[J].激光生物学报,2025,34(5):459-465,7.基金项目
河北省邯郸市科学技术研究与发展计划项目(1623208053ZC) （1623208053ZC）
2024年国家医药卫生科技发展研究中心课题(W2024ZT139). （W2024ZT139）
