激光生物学报2025，Vol.34Issue(5)：473-480,8.DOI:10.3969/j.issn.1007-7146.2025.05.011
lncRNA BCAN-AS1/miR-1224-5p/Wnt轴调控舌鳞状细胞癌细胞增殖和侵袭的机制研究
A Study on the Mechanism of lncRNA BCAN-AS1/miR-1224-5p/Wnt Axis Regulating Proliferation and Invasion of Tongue Squamous Cell Carcinoma Cells
摘要
Abstract
To investigate the expression of long noncoding RNA(lncRNA)BCAN-AS1 in tongue squamous cell carcinoma(TSCC)and the mechanism by which BCAN-AS1/miR-1224-5p/Wnt axis regulates the proliferation and invasion of TSCC cells,cBioPortal database was used to analyze BCAN-AS1 expression in TSCC tissues.qRT-PCR was used to detect the expression of BCAN-AS1 in normal human oral keratinocytes(HOK)and TSCC cells(Tca8113,HNl3,SCC-9,TSCCA,SCC-15).SCC-9 cells were cultured in vitro and divided into Con group(transfected with negative plasmid)and si-BCAN-AS1 group(transfected with si-BCAN-AS1 plasmid)according to the treatment method.The proliferation and invasion abilities of SCC-9 cells in each group were detected by clone formation assay and Transwell assay.Dual luciferase reporter assay verified the complementary combination between BCAN-AS1 and miR-1224-5p.The cBioPortal database was used to analyze the correlation between BCAN-AS1 and miR-1224-5p expressions in TSCC tissues.qRT-PCR was used to detect miR-1224-5p expression in SCC-9 cells in each group.Western blot detected Wnt/β-catenin pathway proteins expression in SCC-9 cells in each group.Compared with adjacent tissues,BCAN-AS1 was highly expressed in TSCC tissues(P<0.01).Compared with HOK cells,BCAN-AS1 was highly expressed in Tca8113,HNl3,SCC-9,TSCCA,and SCC-15 cells(P<0.01).The cell proliferation and invasion abilities of the si-BCAN-AS1 group were lower than those of the Con group(all P<0.01).BCAN-AS1 could target and bind to miR-1224-5p(P<0.01).miR-1224-5p expression in SCC-9 cells increased(P<0.01),and the expressions of β-catenin,Cyclin D1,с-mус,and MMP-7 proteins decreased(all P<0.01).BCAN-AS1 is highly expressed in TSCC,and downregulation of BCAN-AS1 can regulate the proliferation and invasion of TSCC SCC-9 cells through the miR-1224-5p/Wnt/β-catenin axis.BCAN-AS1 may become a molecular target for targeted therapy of TSCC.关键词
BCAN-AS1/舌鳞状细胞癌/口腔角质细胞/miR-1224-5p/Wnt/β-catenin通路Key words
BCAN-AS1/tongue squamous cell carcinoma/oral keratinocytes/miR-1224-5p/Wnt/β-catenin pathway分类
临床医学引用本文复制引用
王瑾,廖立凡,彭欣,李曦雯,朱瑶琪,王浩..lncRNA BCAN-AS1/miR-1224-5p/Wnt轴调控舌鳞状细胞癌细胞增殖和侵袭的机制研究[J].激光生物学报,2025,34(5):473-480,8.基金项目
国家自然科学基金项目(82003217). （82003217）
