吉林农业大学学报2025，Vol.47Issue(5)：819-832,14.DOI:10.13327/j.jjlau.2025.0025
转录组分析揭示MS6调控大豆花药发育的分子功能
Transcriptome Analysis Revealed the Molecular Function of MS6 in Regulating Soybean Anther Development
摘要
Abstract
Utilization of heterosis is an effective approach to increase crop yields,with male sterile lines serving as important basic materials due to the decisive role of sterile genes.In order to explore the transcriptional regulatory mechanism of the soybean nuclear male sterile gene MS6(GmTDF1-1),a comparative analysis of the transcriptomes of male sterile plants(ms6)and fertile plants(MS6)was performed using RNA sequencing technology(RNA-seq)to investigate its male sterility mechanism.A total of 625 differentially expressed genes(DEGs)were identified through K-means clustering analysis.GO functional annotation revealed that these DEGs are primarily involved in processes such as external encapsulating structure organization,cell wall organization,molecular function regu-lator,enzyme regulatory activity,and cellular polysaccharide metabolic process.KEGG pathway en-richment analysis indicated that these DEGs are mainly associated with phenylpropanoid and isofla-vonoid biosynthesis,galactose,amino sugar,nucleotide sugar,starch,sucrose metabolism and other processes.Protein-protein interaction(PPI)network analysis,constructed using STRING software,identified direct or indirect interactions between TDF1 and proteins such as DYT1,RD19A,KCS7,KCS21,AMS,MS1,and A6.These findings provide new insights into the molecular mechanism un-derlying soybean male sterility and lay a theoretical foundation for its application in hybrid breeding.关键词
大豆/雄性不育/转录组测序/MS6/核不育Key words
soybean/male sterility/RNA sequencing/MS6/nuclear sterility分类
农业科技引用本文复制引用
刘铭,申明哲,王铭钰,吴松权,林春晶,刘璐,方小龙,张春宝..转录组分析揭示MS6调控大豆花药发育的分子功能[J].吉林农业大学学报,2025,47(5):819-832,14.基金项目
吉林省科技发展计划项目(20240101214JC),国家大豆产业技术体系建设专项(CARS-04),吉林省农业科技创新工程项目(CXGC2023RCY044) （20240101214JC）
