吉林农业大学学报2025，Vol.47Issue(5)：864-875,12.DOI:10.13327/j.jjlau.2022.1784
大豆Dof类转录因子家族生物信息学分析及油脂合成相关GmDof4基因的克隆
Bioinformatics Analysis of Soybean Dof Transcription Factor Family and Cloning of GmDof4 Genes Related to Oil Synthesis
摘要
Abstract
Dof-class transcription factors,a class of plant transcription factors with specific con-served domains,receive increasingly much academic attention for their important role in plant meta-bolic regulation,root development,gene defense regulation and other processes.Based on the previ-ous transcriptomic sequencing analysis of soybean hypolinolenic acid mutants,33 soybean Dof tran-scription factors with high expression level were selected,and the physicochemical properties,kin-ship and protein interaction analysis of the encoded proteins were performed;to further determine the function of Dof4 interacting with the acetyl-CoA carboxylase(accD)catalyzing oil synthesis,the plant expression vector of GmDof4 gene was cloned and constructed,and the promoter element and bind-ing site prediction were analyzed against the 2 000 bp sequence upstream of the start codon of Gm-Dof4 gene.The results showed that the 33 GmDof transcription factors all contained the specific mo-tifs CX2CX21CX2C and were unevenly distributed across 15 chromosomes in soybean;The proteins encoded by these transcription factors were predicted to be localized in the nucleus;Among the 28 predicted protein-protein interactions,43%of the Dof transcription factors were directly or indirectly closely related to insect resistance,cold resistance,drought resistance,salinity resistance and seed development in soybean,and only Dof4 showed a direct interaction relationship with key enzymes in oil synthesis;The full-length CDS sequence of the GmDof4 gene was obtained by RT-PCR to suc-cessfully construct the pCAMBIA3301-Dof4 plant overexpression vector.The results of promoter ele-ment and binding site analysis indicate that GmDof4 gene may function together with multiple key enzyme genes in catalytic oil synthesis to affect soybean oil synthesis,in order to lay the preliminary foundation for the regulation of seed oil metabolism by transcription factors in soybean Dof.关键词
大豆/Dof转录因子/生物信息学/启动子元件分析/基因克隆Key words
Glycine max/transcription factor of Dof/information biology/promoter element analy-sis/gene cloning分类
农业科技引用本文复制引用
赵秋竹,栾鑫超,李婷婷,王丽雪,徐作成,李越,姚丹..大豆Dof类转录因子家族生物信息学分析及油脂合成相关GmDof4基因的克隆[J].吉林农业大学学报,2025,47(5):864-875,12.基金项目
吉林省科技发展计划项目(20210202006NC) （20210202006NC）
