摘要
Abstract
Objective:To investigate whether artesunate affects the proliferation,migration and invasion of thyroid cancer cells through the circ_0085616/miR-338-3p pathway.Methods:RT-qPCR method was used to detect the expression of circ_0085616 and miR-338-3p in thyroid cancer tissues and adjacent non-cancerous tissues.Pearson correlation method was used to analyze the correlation between circ_0085616 and miR-338-3p expressions in thyroid cancer tissues.Human thyroid cancer SW579 cells were cultured in vitro and treated with different doses of artesunate.Cells were transfected with si-NC,si-circ_0085616,pcDNA,or pcDNA-circ_0085616.After transfection of pcDNA or pcDNA-circ_0085616,cells were treated with 90 μmol/L artesunate.CCK-8 method,plate colony formation experiment,Transwell experiment,and Western blotting assay were used to detect proliferation,colony formation,migration,invasion,and protein expression changes of SW579 cells,respectively.The dual luciferase reporter experiment,RNA pull-down assay and RNA immunoprecipitation assay were used to verify the targeting relationship between circ_0085616 and miR-338-3p.Results:The expression of circ_0085616 in thyroid cancer tissues was higher than that in adjacent tissues,while the expression of miR-338-3p was lower than that in adjacent tissues.A negative correlation was observed between circ_0085616 and miR-338-3p expression.Artesunate reduced the expression of circ_0085616 and the protein level of N-cadherin,decreased the number of colony formation,migrating and invading cells,while increasing the inhibition rate of cell proliferation,the expression of miR-338-3p and the protein level of E-cadherin.circ_0085616 targeted the expression of miR-338-3p.Transfection of si-circ_0085616 inhibited cell proliferation,clone formation,migration and invasion,while transfection of pcDNA-circ_0085616 attenuated the inhibitory effects of artesunate on the proliferation,clone formation,migration and invasion of SW579 cells.Conclusion:Artesunate inhibits the proliferation,migration and invasion of thyroid cancer cells by regulating the expression of circ_0085616/miR-338-3p.关键词
甲状腺癌/青蒿琥酯/circ_0085616/miR-338-3p/细胞增殖/迁移/侵袭Key words
thyroid cancer/artesunate/circ_0085616/miR-338-3p/cell proliferation/migration/invasion分类
医药卫生
