摘要
Abstract
Objective:To investigate the effects of isorhamnetin(ISO)on proliferation,invasion,and apoptosis of esophageal cancer cells and its mechanism of action.Methods:Human esophageal cancer EC109 cells were divided into control group,low,medium,and high concentration isorhamnetin group(ISO-L,ISO-M,ISO-H group),and ISO-H+KI696 group.CCK-8 assay was applied to detect the proliferation ability.Transwell assay was utilized to determine their invasive ability.Flow cytometry was employed to measure their apoptosis rate.RT-qPCR method was adopted to detect the mRNA expression levels of p62,Kelch-like ECH associated protein 1(Keap1),and nuclear factor erythroid 2-related factor 2(Nrf2).Western blotting was used to detect the expression levels of p62,Keap1,and Nrf2 proteins.Results:Compared with the control group,the ISO-treated groups exhibited significantly reduced proliferation and invasion abilities,lower levels of p62 and Nrf2 mRNA and proteins,while showing higher apoptosis rates and mRNA and protein levels of Keap1.These effects were dose-dependent.However,KI696,an inhibitor of the Keap1-Nrf2 axis,reversed the inhibitory effects of ISO on cell proliferation and invasion.Conclusion:ISO inhibits the proliferation and invasion of esophageal cancer cells,and promotes their apoptosis by downregulating p62 expression and enhancing Keap1-mediated inhibition of Nrf2 signaling.关键词
异鼠李素/食管癌/p62/Kelch样ECH相关蛋白1/核因子红细胞相关因子2/增殖/侵袭Key words
isorhamnetin/esophageal cancer/p62/Keap1/Nrf2/proliferation/invasion分类
医药卫生
