解剖学杂志2025，Vol.48Issue(5)：422-426,5.DOI:10.3969/j.issn.1001-1633.2025.05.011
瑞芬太尼调节HMGB1-RAGE信号通路对神经病理性疼痛大鼠脊髓背角神经元损伤的影响
Effects of remifentanil on spinal cord dorsal horn neuron damage in neuropathic pain rats by regulating the HMGB1-RAGE signaling pathway
摘要
Abstract
Objective:To investigate the effect of remifentanil(REM)on spinal cord dorsal horn neuron injury in rats with neuropathic pain by regulating the high mobility group box 1(HMGB1)-receptor for advanced glycation end products(RAGE)signaling pathway.Methods:SD rats were divided into sham surgery group,model group,low-dose remifentanil(REM-L)group,high-dose remifentanil(REM-H)group,and REM-H+rHMGB1(recombinant protein HMGB1)group.A neuropathic pain rat model was established by spinal nerve ligation.The pain behavior of rats,the levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,and IL-10,histopathological damage and neuronal apoptosis in the spinal dorsal horn,the expression of HMGB1 mRNA and RAGE mRNA,as well as the levels of HMGB1,RAGE,nuclear factor-κB(NF-κB),phosphorylated NF-κB(p-NF-κB),B-cell lymphoma-2(Bcl-2),and Bcl-2 associated X protein(Bax)proteins were detected.Results:After the REM-L and REM-H treatments,the paw retraction threshold and thermal sensitivity latency,IL-10,and Bcl-2 expression were increased,while the expression of TNF-α and IL-1β,apoptosis rate of neurons,HMGB1 and RAGE mRNA and protein expression,the protein expression of p-NF-κB/NF-κB and Bax were reduced.The recombinant protein rHMGB1 was able to partially reverse the therapeutic effect of REM on neuropathic pain in rats.Conclusion:REM can alleviate the spinal dorsal horn nerve tissue damage in rats with neuropathic pain,which may be related to the inhibition of HMGB1-RAGE signaling pathway.关键词
瑞芬太尼/高迁移率族蛋白-B1-晚期糖基化终产物受体信号通路/神经病理性疼痛/脊髓/背角神经元Key words
remifentanil/high mobility group box 11-receptor for advanced glycation end products signaling pathway/neuropathic pain/spinal cord/dorsal horn neuron分类
医药卫生引用本文复制引用
罗一帆,邵琛,卢俊..瑞芬太尼调节HMGB1-RAGE信号通路对神经病理性疼痛大鼠脊髓背角神经元损伤的影响[J].解剖学杂志,2025,48(5):422-426,5.基金项目
十堰市2021年引导性科研项目(21Y41) （21Y41）
