江苏农业学报2025，Vol.41Issue(10)：1886-1898,13.DOI:10.3969/j.issn.1000-4440.2025.10.002
大豆TOR家族全基因组鉴定及表达特征分析
Genome-wide identification and expression characteristics analysis of soy-bean TOR family
摘要
Abstract
The target of rapamycin(TOR)protein serves as a central regulator in plant growth,development,and stress responses.Current research on plant TOR genes remains limited,with particularly scarce investigations in important crops like soybean.This study employed bioin-formatics approaches to identify the soybean TOR gene family and analyzed various characteristics including pro-tein physicochemical properties,gene structure,protein secondary structure,phosphorylation sites,chromosomal localization,phylogenetic relationships,evolutionary constraints,promoter elements,protein interaction networks,and ex-pression patterns.Results revealed that both GmTOR1 and GmTOR2 proteins contain 2 468 amino acids,with secondary structures predominantly composed of α-helices and random coils,exhibiting 193 and 191 high-potential phosphorylation sites,respectively.GmTOR1 and GmTOR2 are located on chromosomes 1 and 11,respectively.Promoter regions contain re-sponse elements for abscisic acid(ABA),methyl jasmonate(MeJA),ethylene(ETH),gibberellin(GA),and salicylic acid(SA),suggesting GmTOR1 and GmTOR2 may participate in hormone regulation and responses to drought stress,salt stress,cold stress,and Phytophthora sojae infection.Transcriptomic data analysis demonstrated that GmTOR1 and GmTOR2 exhibited predominant expression in shoot apices,flowers,lateral buds,and pods.Under flooding stress,their expression in roots decreased initially and then increased,whereas the opposite pattern was observed in the leaves.GmTOR1 and Gm-TOR2 were down-regulated in response to cold stress,Phytophthora sojae infection,and ethylene induction.qRT-PCR vali-dation results indicated that the expression patterns of GmTOR1 and GmTOR2 under salt stress were largely consistent with the transcriptome sequencing results.Here,we systematically analyzed the sequence characteristics,evolutionary relation-ships,and expression patterns of GmTOR1 and GmTOR2.These findings enrich our understanding of the soybean TOR gene family and provide a solid theoretical foundation for their subsequent functional studies.关键词
大豆/GmTOR1/GmTOR2/生物信息学/进化/表达特征Key words
soybean/GmTOR1/GmTOR2/bioinformatics/evolution/expression characteristics分类
农业科技引用本文复制引用
张中起,代名扬,李昆仑,吕令华,刘磊,曹嵩晓,李品,张震彪,薛墨庸..大豆TOR家族全基因组鉴定及表达特征分析[J].江苏农业学报,2025,41(10):1886-1898,13.基金项目
山东省蔬菜产业技术体系菏泽综合试验站项目(SDAIT-05-26) （SDAIT-05-26）
山东省自然科学基金项目(ZR2024QC237) （ZR2024QC237）
