江苏农业学报2025，Vol.41Issue(10)：1920-1925,6.DOI:10.3969/j.issn.1000-4440.2025.10.005
核型多角体病毒SfMNPY-Yz01对草地贪夜蛾解毒酶与防御酶活性的影响
Effects of nucleopolyhedrovirus SfMNPY-Yz01 on the activities of detoxifying enzymes and defensive enzymes in Spodoptera frugiperda
摘要
Abstract
In this study,larvae of Spodoptera frugiperda were infected with the SfMNPV-Yz1 strain of Spodoptera fru-giperda nucleopolyhedrovirus.The dynamic changes in the activities of six key detoxifying and defensive enzymes in the lar-vae during the infection process were systematically determined,including phenol oxidase(PO),glutathione S-transferase(GST),carboxylesterase(CarE),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD).The results showed that during the infection period,the activity of PO fluctuated drastically,which might be associated with the virus regulating the melanization response in insects.Glu-tathione S-transferase(GST)maintained high activity con-sistently,suggesting its role in alleviating oxidative stress and metabolizing toxic substances.In the late stage of in-fection,the activities of catalase(CAT)and peroxidase(POD)increased significantly,indicating their involve-ment in scavenging excessive reactive oxygen species(ROS)to maintain cellular homeostasis.This study clarifies the multi-level physiological responses of Spodoptera frugiperda larvae to baculovirus infection from the perspective of enzyme activity,providing a theoretical basis for the biological control of Spodoptera frugiperda.关键词
草地贪夜蛾/核型多角体病毒/解毒酶/防御酶Key words
Spodoptera frugiperda/nucleopolyhedrovirus/detoxifying enzymes/defensive enzymes分类
农业科技引用本文复制引用
刘琴,徐健,张琥,夏杨,林曼曼,韩光杰,李传明,张楠,陆玉荣,黄立鑫..核型多角体病毒SfMNPY-Yz01对草地贪夜蛾解毒酶与防御酶活性的影响[J].江苏农业学报,2025,41(10):1920-1925,6.基金项目
江苏省农业科技自主创新基金项目(CX-23-3004) （CX-23-3004）
农业基础性长期性科技工作项目(NAES069AM04、NAES-EE-041) （NAES069AM04、NAES-EE-041）
扬州市生态农业重点实验室项目(YZ2023244) （YZ2023244）
