江苏农业学报2025，Vol.41Issue(10)：1926-1935,10.DOI:10.3969/j.issn.1000-4440.2025.10.006
辣椒胶孢炭疽菌中糖苷水解酶蛋白的找寻及生物信息学分析
Finding and bioinformatic analysis of glycoside hydrolase proteins in Colle-totrichum gloeosporioides of pepper
摘要
Abstract
Pepper anthracnose,caused by Colletotrichum gloeosporioides,is a major disease that restricts the high-quality development of the pepper industry.Clarifying the characteristics and functions of glycoside hydrolases(GHs)in this pathogen is of great significance for deciphering its pathogenic mechanism.In this study,bioinformatic methods were used to predict the GH proteins in Colletotrichum gloeosporioides isolated from pepper.Online tools including ProtParam,ProtScale,and SMART were employed to analyze the physicochemical properties,hydrophobicity,and conserved domains of these proteins.MEGA 11 software was utilized to construct a phylogenetic tree for analyzing genetic relationships,while the EggNOG-mapper tool was applied for functional annotation of orthologous group(COG)pro-teins.The results showed that a total of 24 GH proteins were predicted in this pathogen.Most of these proteins were acidic,stable,and hydrophobic,with amino acid lengths ranging from 201 aa to 400 aa.Moreover,glycoside hydrolases containing conserved GH domains were mainly classified into the GH28 subfamily.Based on the phylogenetic tree,the 24 glycoside hydrolases were classified into three major categories.COG functional annotation indicated that these enzymes were primarily involved in the transport and metabolism of carbohydrates,and also played roles in biological processes such as post-translational modification of proteins and chaperone protein regulation.This study clarifies the num-ber,physicochemical properties,and functional localization of glycoside hydrolases in Colletotrichum gloeosporioides of pep-per.It provides important theoretical support for in-depth exploration of the molecular pathogenic mechanism of this patho-gen,accurate identification of targets for pathogen control,and development of novel biological control technologies.Mean-while,it holds high practical value for ensuring the safe production of the pepper industry.关键词
辣椒/胶孢炭疽菌/分泌蛋白/糖苷水解酶Key words
pepper/Colletotrichum gloeosporioides/secreted protein/glycoside hydrolase分类
农业科技引用本文复制引用
龚凤珍,吕俊婷,侯朝英,韩长志..辣椒胶孢炭疽菌中糖苷水解酶蛋白的找寻及生物信息学分析[J].江苏农业学报,2025,41(10):1926-1935,10.基金项目
云南省"兴滇英才支持计划"青年人才专项(YNWR-QN-BJ-2020-188) （YNWR-QN-BJ-2020-188）
云南省研究生导师团队建设项目(2022100) （2022100）
西南林业大学林学学科和重点实验室开放基金项目(LXXK-2025M06) （LXXK-2025M06）
