江苏农业学报2025，Vol.41Issue(10)：1962-1972,11.DOI:10.3969/j.issn.1000-4440.2025.10.010
秸秆还田配施氮肥对冬油菜根际土壤微生物群落的影响
Effects of straw returning combined with nitrogen fertilizer application on the rhizosphere soil microbial community of winter rapeseed
摘要
Abstract
To investigate the effects of fertilization and straw returning on rhizosphere soil nutrients,yield,and mi-crobial community characteristics of winter rapeseed,this study set up four treatments,namely:no straw application and no nitrogen fertilizer application(CK),straw application treatment(T1),nitrogen fertilizer application treatment(T2),and combined application of straw and nitrogen fertilizer treatment(T3).Using Illumina high-throughput sequencing technolo-gy,the bacterial 16S rRNA gene and fungal ITS region were sequenced.A systematic analysis was conducted on the α-di-versity and community structure of bacterial and fungal communities in winter rapeseed rhizosphere soil,as well as their re-lationships with environmental factors.Meanwhile,soil physicochemical properties and winter rapeseed yield were determined synchronously.The results showed that the rapeseed yield and soil fungal community abundance in the T3 treatment were significantly higher than those in the CK,T1,and T2 treatments(P<0.05).The T3 treatment led to the enrichment of fungal communities such as Phae-osphaeria and Cladosporium in the soil.Most fungi of these two genera are pathogenic microorganisms,which may cause rapeseed diseases.Network analysis of environmental factors and microbial communities revealed that four environmental factors(soil alkali-hydrolyzable nitrogen content,total nitrogen content,organic carbon content,and available phosphorus content)exhibited stronger associations with the relative abun-dance of both bacterial and fungal communities.The soil alkali-hydrolyzable nitrogen content was significantly positively cor-related with the relative abundances of bacteria of the genera Arthrobacter and Flavobacterium(P<0.05).Bacteria of these genera may be involved in the crop nitrogen transformation process.The findings of this study provide a theoretical basis for the rational utilization of straw resources,improvement of soil quality,and increase of crop yield.关键词
秸秆还田/微生物群落结构/冬油菜/环境因子Key words
straw return/microbial community structure/winter rapeseed/environmental factors分类
农业科技引用本文复制引用
刘东海,梁思威,张智,肖卓熙,乔艳,任健,赵书军,胡诚..秸秆还田配施氮肥对冬油菜根际土壤微生物群落的影响[J].江苏农业学报,2025,41(10):1962-1972,11.基金项目
国家农业科技项目(20221805) （20221805）
湖北省科技重大专项(2023BBA003) （2023BBA003）
国家重点研发计划项目(2024YFD1-900100) （2024YFD1-900100）
