基于GBS技术的贵州铜仁地方茶树资源遗传关系研究OA北大核心

To provide reliable evidence for origin and evolution,germplasm innovation,selective breeding,reasonable protection and utilization of genetic resources of local tea germplasm,genotyping by sequencing(GBS)technology was applied to identify single nucleotide polymorphism(SNP)loci for 60 local tea germplasms resources from five populations in Tongren city of Guizhou province,and subsequently,phylogenetic trees,principal component analysis(PCA),and population structure analysis were performed to evaluate the genetic relationships of these local tea germplasms resources based on the obtained SNP loci.The results showed that:After screening with parameters of minor allele frequency＞0.05 and data integrity＞0.80,a total of 6 749 675 SNP loci were retained,and the average of integrity-ratio was 92.75％.According to the phylogenetic tree,principal component analysis and population structure analysis,samples from the same region exhibited closer genetic relationships,and 60 local tea germplasms resources were clustered into two clusters:samples of Dejiang and Yanhe were clustered into a cluster,samples of Yinjiang and Shiqian were clustered into a cluster,suggesting distinct ancestral origins.