扬州大学学报（农业与生命科学版）2025，Vol.46Issue(5)：23-30,8.DOI:10.16872/j.cnki.1671-4652.2025.05.003
基于GBS技术的贵州铜仁地方茶树资源遗传关系研究
Population genetic relationships of local tea germplasms resources in Tongren city of Guizhou province based on genotyping by sequencing
摘要
Abstract
To provide reliable evidence for origin and evolution,germplasm innovation,selective breeding,reasonable protection and utilization of genetic resources of local tea germplasm,genotyping by sequencing(GBS)technology was applied to identify single nucleotide polymorphism(SNP)loci for 60 local tea germplasms resources from five populations in Tongren city of Guizhou province,and subsequently,phylogenetic trees,principal component analysis(PCA),and population structure analysis were performed to evaluate the genetic relationships of these local tea germplasms resources based on the obtained SNP loci.The results showed that:After screening with parameters of minor allele frequency＞0.05 and data integrity＞0.80,a total of 6 749 675 SNP loci were retained,and the average of integrity-ratio was 92.75％.According to the phylogenetic tree,principal component analysis and population structure analysis,samples from the same region exhibited closer genetic relationships,and 60 local tea germplasms resources were clustered into two clusters:samples of Dejiang and Yanhe were clustered into a cluster,samples of Yinjiang and Shiqian were clustered into a cluster,suggesting distinct ancestral origins.关键词
茶树资源/简化基因组测序/SNP/遗传结构Key words
tea germplasms resources/genotyping by sequencing/SNP/genetic structure分类
农业科学引用本文复制引用
李龙,刘敏,谭廷鸿,罗静,戴婧婧..基于GBS技术的贵州铜仁地方茶树资源遗传关系研究[J].扬州大学学报（农业与生命科学版）,2025,46(5):23-30,8.基金项目
贵州省科技厅农业攻关项目[NY(2015)3011-1] （2015）
贵州省科技支撑计划项目(20201Y065) （20201Y065）
贵州省科技计划项目(黔科合基础-[2024]青年008) （黔科合基础-[2024]青年008）
