扬州大学学报（农业与生命科学版）2025，Vol.46Issue(5)：31-38,8.DOI:10.16872/j.cnki.1671-4652.2025.05.004
鸽Viperin基因克隆及生物信息学功能分析
Cloning and bioinformatic functional analysis of the pigeon Viperin gene
摘要
Abstract
To investigate the gene structure and predict the biological function of the pigeon endoplasmic reticulum-as-sociated,interferon-inducible antiviral protein(Viperin),nest PCR was used to obtain the pigeon Viperin coding se-quence(CDS)in this study.The sequence characteristics,structural domains,phylogenetic relationships,and potential functions were analyzed by bioinformatics methods.The results indicated that the pigeon Viperin CDS spanned 1 077 bp,encoding a 358 amino-acid protein classified as unstable.The Viperin lacked a signal peptide,exhibited higher hydrophi-licity in the central region,and potentially contained a N-terminal transmembrane domain.Secondary structure analysis of Viperin revealed a composition of 33％α-helices and 13％β-sheets.Tertiary structural modeling of Viperin further dem-onstrated a conserved barrel-shaped architecture characterized by a-helices enveloping β-sheets.Multisequence alignment analysis of Viperin indicated high conservation across species,except in the N-terminal region.Protein interaction net-work analysis of Viperin identified functional associations with key immune regulators,including CMPK2,IRF1,and TRAF6.Collectively,these results will provide a theoretical basis for functional studies of pigeon Viperin.关键词
鸽/Viperin/克隆/生物信息学Key words
pigeon/Viperin/cloning/bioinformatics分类
畜牧业引用本文复制引用
王鑫,朱奕锟,黄宇飞,杨盛..鸽Viperin基因克隆及生物信息学功能分析[J].扬州大学学报（农业与生命科学版）,2025,46(5):31-38,8.基金项目
江苏省研究生实践创新计划项目(SJCX24_2318) （SJCX24_2318）
江苏省高校基础科学(自然科学)研究基金资助项目(23KJB230006) （自然科学）
