扬州大学学报（农业与生命科学版）2025，Vol.46Issue(5)：56-65,10.DOI:10.16872/j.cnki.1671-4652.2025.05.007
lncRNA JPX通过m6A-YTHDC2轴上调细胞质p21蛋白促进RA-FLS增殖
lncRNA JPX contributes to RA-FLS proliferation by up regulating the cytoplasmic p21 protein via m6A-YTHDC2 axis
摘要
Abstract
The aim of the present study was to reveal the underlying molecular mechanism by which lncRNA JPX regu-lated the activity of rheumatoid arthritis fibroblast-like synoviocytes(RA-FLS).The reverse transcription-quantitative re-al-time polymerase chain reaction(RT-qPCR),Western blot,methylated RNA immunoprecipitation(MeRIP)assay,lu-ciferase reporter assay,RNA immunoprecipitation(RIP)assay,protein stability assay,and in vitro functional experi-ments were performed to explore the role and molecular mechanism of JPX in regulating RA-FLS activation.The results showed that JPX increased the N6-methyladenosine(m6A)modification in p21 mRNA,which increased its interaction with YTHDC2 to upregulate the expression of p21 protein in RA-FLS.Meanwhile,JPX further increased the enrichment of p21 protein in the cytoplasm of RA-FLS and subsequently promoted their proliferation.In summary,JPX can regulate RA-FLS proliferation through an m6 A-YTHDC2-p21 axis and the distribution of p21 protein in cells.JPX might be a no-vel target for RA diagnosis and treatment.关键词
lncRNA JPX/类风湿关节炎成纤维细胞样滑膜细胞(RA-FLS)/p21/N6甲基腺苷(m6A)/含YTH结构域蛋白2(YTHDC2)Key words
lncRNA JPX/rheumatoid arthritis fibroblast-like synoviocytes(RA-FLS)/p21/N6-methyladenosine(m6A)/YTH domain containing 2(YTHDC2)分类
临床医学引用本文复制引用
韦丽丽,杨洁,沈维干,周玮..lncRNA JPX通过m6A-YTHDC2轴上调细胞质p21蛋白促进RA-FLS增殖[J].扬州大学学报（农业与生命科学版）,2025,46(5):56-65,10.基金项目
国家自然科学基金资助项目(82101890) （82101890）
