扬州大学学报（农业与生命科学版）2025，Vol.46Issue(5)：66-74,134,10.DOI:10.16872/j.cnki.1671-4652.2025.05.008
修饰氯喹脂质体的制备及其对外泌体分泌的影响
Preparation of modified chloroquine liposomes and its infuluence on exosome secretion
摘要
Abstract
To investigate the effects of chloroquine liposomes(CQ-Lips)modified with cationic materials and cell-pene-trating peptides(CPPs)on cellular exosome secretion,modified CQ-Lips composed of soybean phosphatidylcholine,cho-lesterol,DOTAP,DOPC,and TAT peptide were prepared using freeze-thaw cycles.The cytotoxicity of cationic materials with varying charge ratios[DOTAP/DOPC(5∶0)-(1∶4)],concentration gradients(7.5,15,30 mmol·L-1),andTAT modification levels(1％-9％)was evaluated vriaCCK-8 assay.Western blot was employed to quantify exosome secretion under different modification conditions and identify optimal parameters.Results demonstrated that cationic materials ex-hibited concentration-dependent cytotoxicity,with the 30 mmol·L-1 group showing significantly reduced cell viability(P＜0.01),while no statistical differences were observed among groups with the same concentration(15 mmol·L-1)and var-ying charge ratios.TAT modification at concentrations＞5％significantly decreased cell viability(P＜0.01).The dual-modified CQ-Lips(15 mmol·L-1 cationic material,DOTAP/DOPC 5∶0;5％TAT)exhibited superior exosome secre-tion compared to single-modified groups(P＜0.05).In conclusion,this study successfully established a novel TAT-cationic co-modified CQ-Lips delivery system,which significantly enhanced exosome secretion by optimizing charge parameters and CPP ratios,providing critical experimental insights for advancing high-efficiency exosome production technologies.关键词
脂质体/外泌体产量/氯喹/阳离子衍生物/细胞穿膜肽Key words
liposomes/exosome yield/chloroquine/cationic derivatives/cell-penetrating peptides(CPPs)分类
药学引用本文复制引用
刘杰峰,晏兰兰,王学丽,和卓怡,冯光涯,马波,鲁卫东..修饰氯喹脂质体的制备及其对外泌体分泌的影响[J].扬州大学学报（农业与生命科学版）,2025,46(5):66-74,134,10.基金项目
云南省重点研发计划项目(202003AC100015) （202003AC100015）
昆明医科大学研究生创新基金项目(2024S019) （2024S019）
