扬州大学学报（农业与生命科学版）2025，Vol.46Issue(5)：82-95,14.DOI:10.16872/j.cnki.1671-4652.2025.05.010
基于UPLC-Q-TOF-MS/MS技术解析温经汤基准样品成分
Analysis of chemical composition of Wenjing Decoction based on UPLC-Q-TOF-MS/MS
摘要
Abstract
The UPLC-Q-TOF-MS/MS method was used to analyze the chemical composition of Wenjing Decoction,and the separation was carried out on a Kromasil 100-1.8-C18 column with a gradient elution of acetonitrile-0.05％formic acid water solution at a volume flow rate of 0.4 mL·min-1 and a column temperature of 35 ℃.ESI was used with both positive and negative ionization modes,and the composition was identified and confirmed by analyzing the accurate molec-ular weight and the mass fragmentation pattern by UPLC-Q-TOF-MS/MS as well as comparing with the multistage mass spectrometric data from the standard substances,software prediction and literature data.A total of 75 chemical compo-nents were identified from the benchmark sample(extracting solution)of Wenjing Decoction,including 28 saponins,16 terpenoids,13 flavonoids,5 phenolic acids,5 phthalides,3 ketosteroid and 5 other compounds,and all of the components were present in relevant herbs.In conclusion,UPLC-Q-TOF-MS/MS enables a rapid,comprehensive method for system-atic qualitative analysis of the chemical constituents in the extracting solution of Wenjing Decoction,which can provide a basis for clarifying the effective substances and improving the level of quality control.关键词
温经汤/基准样品/成分解析/UPLC-Q-TOF-MS/MSKey words
Wenjing Decoction/benchmark sample/chemical composition/UPLC-Q-TOF-MS/MS分类
农业科技引用本文复制引用
黄朝情,刘洋,华杰,曹晖,戴德雄..基于UPLC-Q-TOF-MS/MS技术解析温经汤基准样品成分[J].扬州大学学报（农业与生命科学版）,2025,46(5):82-95,14.基金项目
丽水市经济技术开发区重点研发计划项目(2022KFQZDYF05) （2022KFQZDYF05）
