GA3和CPPU处理对葡萄果实果柄结构的影响OA北大核心

The fruit pedicel serves as a critical conduit for the transport of water and nutrients to the fruit.Gibberellins acid(GA)and the forchlorfenuron(CPPU)are commonly used to enhance grape berry size.To investigate the effects of GA and CPPU on grape pedicel structure and water absorption,clusters of Pinot Noir and Einset Seedless grape varieties were dipped at 14 days after full bloom with the following treatments:clear water(control,CK),25 mg·L-1 GA3,5 mg·L-1 CPPU,and 25 mg·L-1 GA3+5 mg·L-1 CPPU.Changes in fruit size,pedicel structure,and water absorption were measured during the hard-seed stage,veraison and maturity stage.The results showed that all three treatments resulted in increased grape fruit size,pedicel diameter and the xylem and phloem areas of the pedicel vascular bundle.The com-bined 25 mg·L-1 GA3 and 5 mg·L-1 CPPU treatment exhibited the most pronounced effect,and increased the fruit weight of Pinot Noir at maturity by 23.3％,pedicel thickness by 76.9％,and the area of xylem and phloem in the vascu-lar bundles by 47.9％and 6.7％,respectively.For Einset Seedless at maturity,it increased fruit weight by 52.7％,ped-icel thickness by 32.3％,and the area of xylem and phloem in the vascular bundles by 80.2％and 24.9％,respectively.25 mg·L-1 GA3 primarily increased the xylem area,while 5 mg·L-1 CPPU favored an increase in phloem area.Addi-tionally,25 mg·L-1 GA3 and 5 mg·L-1 CPPU treatments increased water absorption in fruit at the green-hard stage and veraison stage,with the order of effectiveness being 25 mg·L-1 GA3+5 mg·L-1 CPPU＞25 mg·L-1 GA3＞5 mg·L-1 CPPU＞CK.In conclusion,GAs and CPPU promoted an increase in the vascular bundle area of fruit pedicels,which could facilitate water transport and subsequently influence fruit expansion.These findings offer a new perspective for further analyzing the mechanisms underlying fruit expansion and provide an important reference for the development of innovative technologies or cultivation practices aimed at enhancing fruit expansion in grape production.