实用心电与临床诊疗2025，Vol.34Issue(5)：659-664,6.DOI:10.13308/j.issn.2097-5716.2025.05.007
鸢尾素通过MEK1/RanBP2信号通路对心肌缺氧复氧损伤的保护作用
Protective effect of irisin against myocardial hypoxia/reoxygenation injury via MEK1/RanBP2 signaling pathway
摘要
Abstract
Objective To investigate whether irisin exerts its protective effect against myocardial hypoxia/reoxygenation(H/R)injury by regulating the MEK1/RanBP2 signaling pathway.Methods An H/R injury model was established by using H9C2 cardiomyocytes.The H9C2 cells were randomly divided into four groups:a control group(untreated H9C2 cells),an H/R group(H9C2 cells subjected to H/R injury),an irisin group(H9C2 cells pretreated with 10 ng/mL irisin for 2 h),and an H/R+irisin group(H9C2 cells pretreated with 10 ng/mL irisin for 2 h followed by H/R injury).Cell viability was assessed using the MTT assay,and cell death rate was evaluated by lactate dehydrogenase leakage rate.Mitochondrial dysfunction induced by H/R injury was also detected.Changes in the expression of the E3 ubiquitin ligase(RanBP)2 and MEK1 signaling molecules were analyzed by immunoblotting.Results Irisin significantly inhibited H/R-induced H9C2 cardiomyocyte death.The cell viability rate was only(43.28±2.37)％in the H/R group,however it increased to(76.24±6.28)％after irisin pretreatment(P=0.000 7),and the cell death rate decreased from(57.38±4.38)％to(26.38±3.29)％(P=0.000 4).Additionally,irisin effectively inhibited the opening of the mitochondrial permeability transition pore induced by H/R injury.Mechanistic studies further revealed that the protective effect of irisin was associated with the regulation of RanBP2 cellular localization via the MEK1 signaling pathway.Conclusion Irisin protects against myocardial H/R injury by inhibiting MEK1-mediated sequestration of RanBP2 and altering the cellular localization of RanBP2.关键词
鸢尾素/心肌细胞/缺氧复氧损伤/急性心肌梗死Key words
irisin/cardiomyocyte/hypoxia/reoxygenation injury/acute myocardial infarction分类
医药卫生引用本文复制引用
王洋洋,王皓,杨泾纬,谷云飞..鸢尾素通过MEK1/RanBP2信号通路对心肌缺氧复氧损伤的保护作用[J].实用心电与临床诊疗,2025,34(5):659-664,6.基金项目
河南省自然科学基金资助项目(232300420247) （232300420247）
