口腔颌面外科杂志2025，Vol.35Issue(5)：365-372,8.DOI:10.12439/kqhm.1005-4979.2025.05.005
组织蛋白酶K对小鼠颌骨骨髓间充质干细胞分化影响的实验研究
Effects of cathepsin K on the differentiation of mouse jaw bone marrow-derived mesenchymal stem cells
摘要
Abstract
Objective:To investigate the effects of cathepsin K(Ctsk)on the proliferation,migration,and differentiation of jaw bone marrow-derived mesenchymal stem cells(JBMMSCs)in mice.Methods:Mouse JBMMSCs were cultured and identified in vitro.Third-passage(P3)cells were subjected to osteogenic,chondrogenic,and adipogenic tri-lineage induction.The expression of Ctsk was detected using real-time quantitative polymerase chain reaction(RT-qPCR).Small interfering RNA(siRNA)transfection was used to knockdown Ctsk expression in JBMMSCs,with knockdown efficiency verified by RT-qPCR and Western blotting.Cell proliferation was assessed using the CCK8 assay,and cell migration was evaluated via scratch wound healing assay.The expression of key genes during tri-lineage differentiation was detected by RT-qPCR.Alkaline phosphatase(ALP)staining was used to assess osteogenic mineralization,Alcian blue staining was applied to evaluate cartilage matrix secretion,and oil red O staining was employed to examine lipid droplet formation.Results:Ctsk expression increased after osteogenic and chondrogenic induction,but decreased after adipogenic induction.Following Ctsk knockdown,cellular migration,osteogenesis,and chondrogenesis were attenuated,but proliferation and adipogenic ability were enhanced.Conclusion:Ctsk promotes the migration,osteogenesis,and chondrogenesis in mouse JBMMSCs,but inhibits their proliferation and adipogenic differentiation.关键词
颌骨骨髓间充质干细胞/组织蛋白酶K/增殖/迁移/分化Key words
jaw bone marrow-derived mesenchymal stem cells/cathepsin K/proliferation/migration/differentiation分类
口腔医学引用本文复制引用
初丹娜,王佐林..组织蛋白酶K对小鼠颌骨骨髓间充质干细胞分化影响的实验研究[J].口腔颌面外科杂志,2025,35(5):365-372,8.基金项目
国家自然科学基金(81670962) （81670962）
国家重点研发计划(2018YFE0202200) （2018YFE0202200）
中央高校基本业务费专项基金(22120180196) （22120180196）
