口腔颌面外科杂志2025，Vol.35Issue(5)：373-381,9.DOI:10.12439/kqhm.1005-4979.2025.05.006
种植体周围组织和牙周组织中Piezo阳性神经纤维分布差异
Differences in the distribution of Piezo-positive nerve fibres between peri-implant and periodontal tissues
摘要
Abstract
Objective:To evaluate the differences in nerve fiber density and the percentage of Piezo-positive nerve fibers between peri-implant and periodontal tissues.Methods:Primary trigeminal ganglion neurons were isolated from male Sprague-Dawley(SD)rats,and the expression of Piezo ion channels in these neurons was confirmed by immunofluorescence staining,real-time quantitative polymerase chain reaction(RT-qPCR)and gel electrophoresis.Subsequently,a dental implant model was established in the maxillary first molar region of male SD rats.One month after osseointegration,the rats were euthanized,and the maxillae were collected.Hematoxylin-eosin(HE)staining and immunofluorescence staining were employed to analyze and compare the differences in nerve fiber density and the percentage of Piezo-positive nerve fibers across four regions between the peri-implant tissues and the periodontal tissues.Results:Immunofluorescence staining,RT-qPCR and gel electrophoresis results demonstrated that both Piezo 1 and Piezo 2 ion channels were expressed in trigeminal ganglion neurons.In both peri-implant tissues and periodontal tissues,the gingival and apical regions exhibited a higher density of nerve fibers,with similar nerve fiber density observed in the gingival region between the two tissue types.In the middle region,the density of nerve fibers in the periodontal ligament was significantly higher than that in the bone surrounding the implant,while no significant differences were found in the gingival,apical and coronal regions.In the gingival region,there were no notable differences in the percentage of Piezo 1-and Piezo 2-positive nerve fibers among the groups.In the coronal and middle root regions,the percentage of Piezo 1-and Piezo 2-positive nerve fibers in the periodontal ligament was significantly higher than that in the alveolar bone directly adjacent to the implant.Conclusion:In the coronal and middle root regions,the percentage of Piezo-positive nerve fibers in the periodontal ligament was significantly higher than that in the alveolar bone directly adjacent to the implant.关键词
Piezo离子通道/神经纤维/种植体/牙齿Key words
Piezo ion channels/nerve fibers/implant/tooth分类
口腔医学引用本文复制引用
陆麒元,魏玉,胡帅,余晶,陶建祥..种植体周围组织和牙周组织中Piezo阳性神经纤维分布差异[J].口腔颌面外科杂志,2025,35(5):373-381,9.基金项目
国家自然科学基金(81970962) （81970962）
上海市卫生健康委员会项目(202340063) （202340063）
