林产化学与工业2025，Vol.45Issue(5)：1-8,8.DOI:10.20195/j.issn.0253-2417.2024146
皂荚皂素-银离子螯合物的构建及其性质研究
Construction and Properties of Gleditsia sinensis Saponin-silver Ion Chelates
摘要
Abstract
In order to enhance the antibacterial activity of Gleditsia sinensis saponin(GSS),the GSS-silver iron chelate was prepared.The optimal preparation process of GSS-Ag was determined through single-factor experiments.The physicochemical properties of GSS-Ag were characterized using Fourier transform infrared spectrometer(FT-IR),scanning electron microscopy(SEM),energy disperse spectroscopy(EDS),and dynamic light scattering nano-particle size analyzer.The foaming capacity and foam stability of GSS-Ag were analyzed.Meanwhile,the antibacterial activity and mechanism of GSS-Ag were investigated.The results indicated that the optimal preparation conditions for GSS-Ag were as follows:pH value of 7,temperature of 50℃,and the m(GSS)∶m(AgNO3)=10∶1.Under these conditions,the chelation capacity of GSS to Ag+reached 7.56 mg/g.The physicochemical property test results indicated that the hydroxyl groups in GSS participated in chelating Ag+without changing the basic chemical structure of GSS.After chelation,Ag+distributed uniformly in GSS,forming a stable chelate.The average particle size of GSS-Ag was 17.94 nm,and the Zeta potential was-6.32 mV,which underwent significant changes compared to the average particle size of 36.70 nm and the Zeta potential of-22.0 mV in GSS,indicating that Ag+had formed a chelate with the negatively charged functional groups(—OH)in GSS.GSS-Ag exhibited excellent foam performance with a foaming capacity of 69.3％at a low mass fraction(0.001％),which was 1.5 times that of Tween 80.Moreover,GSS-Ag showed a good foam stability.The foam stability of GSS-Ag was 61.95％at 240 min,higher than that of Tween 80(51.49％).In addition,the minimum inhibitory mass concentrations(MIC)of GSS-Ag against Escherichia coli and Staphylococcus aureus were 2.5 and 0.625 g/L,respectively,which was significantly lower than that of GSS(both＞100 g/L).The results of alkaline phosphatase(AKP)activity test showed that GSS-Ag could exert antibacterial effects by destroying the bacterial cell wall.关键词
皂素/泡沫稳定性/抑菌Key words
saponin/foam stability/antibacterial分类
化学工程引用本文复制引用
邓叶俊,黄立新,张彩虹,谢普军..皂荚皂素-银离子螯合物的构建及其性质研究[J].林产化学与工业,2025,45(5):1-8,8.基金项目
国家重点研发计划资助项目(2022YFD2200804-3) （2022YFD2200804-3）
