林产化学与工业2025，Vol.45Issue(5)：36-42,7.DOI:10.20195/j.issn.0253-2417.2024104
花椒叶提取物绿色合成纳米银颗粒及其抑菌活性研究
Green Synthesis of Silver Nanoparticles From Zanthoxylum bungeanum Leaf Extracts and Their Antibacterial Activity
摘要
Abstract
Silver nanoparticles(AgNPs)were prepared by green synthesis method with Zanthoxylum bungeanum Maxim.leaf extract as a reducing agent,AgNPs were characterized and analyzed by ultraviolet-visible(UV-Vis)absorption spectrum,Fourier transform infrared spectroscopy(FT-IR),scanning electron microscopy(SEM),X-ray diffraction analysis(XRD),and Zeta potential determination.The results showed that AgNPs had been successfully synthesized,the average particle size of AgNPs was about 50 nm with uniform particle size distribution,showing a spherical face-centered cubic structure with bioactive compounds from Z.bungeanum leaf extracts on the surface.The Zeta potential was(-30.4±0.05)mV,implying good stability.The inhibition zone test,SEM observation of bacterial morphology,fluorescent live and dead bacteria staining and bicinchoninic acid assay(BCA)protein kit were tested to analyze the antibacterial activity.The results showed that the synthetic AgNPs had good inhibitory effect on both Escherichia coli and methicillin-resistant Staphylococcus aureus(MRSA).The inhibitory effect of AgNPs was better for E.coli than that for MRSA.At a concentration of 400 mg/L,the inhibition zone diameters for E.coli and MRSA were(14.67±0.37)mm and(13.67±0.58)mm,respectively.Further analysis suggested that the antibacterial mechanism of AgNPs was mainly to destroy the cell membrane of bacteria,so as to cause the outflow of the cellular contents,leading to the death of bacteria.关键词
花椒叶/纳米银/生物还原法/抑菌性能Key words
Zanthoxylum bungeanum leaf/silver nanoparticles/bioreduction/antibacterial properties分类
化学工程引用本文复制引用
孟圆,刘雨晴,梁硕青,刘玉林..花椒叶提取物绿色合成纳米银颗粒及其抑菌活性研究[J].林产化学与工业,2025,45(5):36-42,7.基金项目
陕西省自然科学基础研究计划资助项目(2023-JC-YB-197) （2023-JC-YB-197）
