林产化学与工业2025,Vol.45Issue(5):79-86,8.DOI:10.20195/j.issn.0253-2417.2024171
油茶果壳提取物的抑菌活性及其在水产养殖中的应用
Antibacterial Activity of Camellia oleifera Shell Extract and Its Application in Aquaculture
摘要
Abstract
The Camellia oleifera shell was extracted using an acid hydrolysis method.Gas chromatography-mass spectrometry(GC-MS)was used to analyze the components of the extract.In vitro,antimicrobial assays were conducted to determine the antibacterial activity of the C.oleifera shell extract against five prevalent pathogenic aquatic bacteria.Additionally,aquaculture experiments were performed to evaluate the effects of the extract on the growth,antioxidant capacity,and immune function of juvenile largemouth bass(Micropterus salmoides).The results revealed that the extract contained esters(15.42%),furans(61.75%),phenols(8.43%),and ketones(3.90%).The extract demonstrated significant antibacterial activity against Vibrio harveyi,Pseudomonas plecoglossicida,Edwardsiella tarda,Aeromonas hydrophila,and V.alginolyticus,exhibiting extreme high sensitivity.After 56 days of feeding experiment,the addition of 0.1%extract in the diet(A1 diet)promoted the growth(weight gain rate reached 441.19%±9.49%)and feed utilization(feed conversion ratio was 1.00±0.01)of juvenile largemouth bass,and enhanced the capacity of antioxidant and immune in juvenile largemouth bass.Specifically,it is manifested as an increase in the activity of serum total superoxide dismutase and the level of total antioxidant capacity,along with an increase in the activity of alkaline phosphatase and the content of immunoglobulin M,and a decrease in malondialdehyde content.关键词
油茶果壳/酸水解提取/抑菌活性/大口黑鲈/促生长Key words
Camellia oleifera shell/Acid hydrolysis extraction/Antibacterial activity/Largemouth bass/Promoted growth分类
化学化工引用本文复制引用
杨曼绮,左元怡,张亮亮,陆黎明,刘义稳,蒋剑春..油茶果壳提取物的抑菌活性及其在水产养殖中的应用[J].林产化学与工业,2025,45(5):79-86,8.基金项目
国家重点研发计划资助项目(2022YFD2200604) (2022YFD2200604)
南平市科技规划项目(N2023Y014) (N2023Y014)