岭南现代临床外科2025，Vol.25Issue(5)：302-311,10.DOI:10.3969/j.issn.1009-976X.2025.05.004
槲皮苷通过JAK2/STAT3信号通路促进小鼠心肌梗死后M2型巨噬细胞极化
Quercitrin promotes M2 macrophage polarization after myocardial infarction in mice through the JAK2/STAT3 signaling pathway
摘要
Abstract
Objective To investigate the effects of quercitrin on ventricular remodeling after myocardial infarction in mice and its underlying mechanisms,providing a basis for the application of quercitrin in cardiovascular diseases.Methods In the in vivo experiment,a mouse model of myocardial infarction was established.Single-cell RNA sequencing technology was used to analyze the immune cell and macrophage subtypes in the myocardial tissue of mice treated with quercitrin.In the in vitro experiment,mouse bone marrow macrophages were isolated and cultured.The polarization state of macrophages was detected after treatment with quercitrin.Western Blot and qPCR were used to detect the protein and gene expression of the JAK2/STAT3 signaling pathway,and the role of the pathway was verified using the JAK inhibitor Ruxolitinib.Results In the in vivo experiment,single-cell RNA sequencing revealed that quercitrin altered the composition of immune cells in the myocardium of mice with myocardial infarction.The total proportion of macrophages increased,with an increase in M2-type macrophage subtypes(such as Macro_Chil1,Macro_Cd163)and a decrease in M1-type(such as Macro_Cd86).In the in vitro experi-ment,flow cytometry showed that quercitrin increased the proportion of M2-type macrophages(P<0.05)and decreased M1-type(P<0.01).Western Blot and qPCR results indicated that the expression of TGF-β1,TGF-β2,VEGF-A,and VEGF-B in mouse macrophages was upregulated after quercitrin treatment(all P<0.001),and this upregulation was reversed by the JAK inhibitor Ruxolitinib.Conclusion Quercitrin promotes M2 macrophage polarization by activating the JAK2/STAT3 signaling pathway,inhibits inflam-matory responses,and promotes angiogenesis,thereby improving ventricular remodeling after myocardial infarction.关键词
心肌梗死/心室重构/槲皮苷/巨噬细胞/单细胞测序Key words
myocardial infarction/ventricular remodeling/quercitrin/macrophages/single-cell sequencing分类
临床医学引用本文复制引用
刘丛勇,傅媛,李婧雯,曾晖,郑俊猛,高敏楠..槲皮苷通过JAK2/STAT3信号通路促进小鼠心肌梗死后M2型巨噬细胞极化[J].岭南现代临床外科,2025,25(5):302-311,10.基金项目
深圳市福田区重点专科建设经费(ZXZX20241061) （ZXZX20241061）
