落叶果树2025,Vol.57Issue(5):35-38,4.DOI:10.13855/j.cnki.lygs.2025.05.008
含水量与植物生长调节剂对苹果砧木组培苗瓶外生根效果的影响
Effects of water content and plant growth regulators on the ex vitro rooting effects of tissue-cultured apple rootstock seedlings
韩秀 1王晓英 1仲凤维 1周光锋 1秦宁 1李国华1
作者信息
- 1. 泰安市泰山林业科学研究院,山东泰安 271000
- 折叠
摘要
Abstract
Taking tissue-cultured seedlings of apple rootstock G935 as the test material,the effects of different water contents and different concentrations of plant growth regulators on the ex vitro rooting effect of tissue-cultured seedlings when using vermiculite as the substrate were explored.The results showed that when the water content of vermiculite was 28%,the rooting rate of tissue-cultured seedlings reached 68.67%,with an average of 11 roots per seedling,the average length of primary lateral roots was 24.60 mm,and the average length of the longest root was 39.64 mm;all these indicators were higher than those when the water content of vermiculite was 8%,18%,24%,and 38%.On the basis of 28%vermiculite water content,dipping the base of seedlings in 4 000 mg/L IAA for 3 seconds achieved the best ex vitro rooting effect on tissue-cultured seedlings,which was better than that of IBA and NAA.After 30 days of cultivation in an artificial climate chamber,the rooting rate of tissue-cultured seedlings reached 97.1%,with an average of 15 roots per seedling,among which the length of primary lateral roots was 44.25 mm,and the length of the longest root was 60.47 mm.关键词
苹果砧木/组培苗/瓶外生根/基质含水量/植物生长调节剂Key words
apple rootstock/tissue-cultured seedlings/ex vitro rooting/substrate water content/growth regulators分类
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韩秀,王晓英,仲凤维,周光锋,秦宁,李国华..含水量与植物生长调节剂对苹果砧木组培苗瓶外生根效果的影响[J].落叶果树,2025,57(5):35-38,4.